| 货号 | 39356 |
| 克隆类别 | 2A9 |
| 同种亚型 | IgG |
| 反应种属 | Mouse |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Western Blot |
| 使用方法 | Validated Applications: ChIP: 4 µg per ChIP ChIP-Seq: 4 µg each WB: 0.5 - 2 µg/ml dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. Published Applications: see references |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Ikaros antibody was raised against a recombinant protein corresponding to the N-terminal half of mouse Ikaros. |
| 背景 | Ikaros is a transcription factor that plays a critical role in the control of lymphohematopoiesis and immune regulation. All Ikaros isoforms share a common C-terminal domain that contains a transcription activation motif and two zinc finger motifs required for hetero- and homo-dimerization among the Ikaros isoforms, and for interactions with other proteins. Ikaros is abundantly expressed in pituitary mammosomatotrophs, where it deacetylates Histone H3 sites on the proximal growth hormone (GH) promoter to silence gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| 计算分子量 | 62 kDa |
| 参考文献 |
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Ikaros antibody (mAb) (Clone 2A9) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse spleen and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Ikaros binding sites. The image shows binding across a region of chromosome 9. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
Ikaros antibody (mAb) (Clone 2A9) tested by Western blot. Detection of Ikaros by Western blot analysis. Ikaros detected in 20 µg mouse spleen whole-cell extract using Ikaros mAb (Clone 2A9) at a 2 µg/ml dilution. |
