| 货号 | 39354 |
| 克隆类别 | 2G1/F7 |
| 同种亚型 | IgG1 |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunoprecipitation/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 0.5 - 2.0 µg/ml dilution Published Applications: IP ICC/IF See references for more information. Individual optimization may be required. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This SIRT1 antibody was raised against a synthetic peptide corresponding to amino acids 581-630 of human SIRT1. |
| 背景 | SIRT1(Sirtuin 1,SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16. SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | 10 mM sodium phosphate pH 7.5, 150 mM NaCl, 30% glycerol, 0.035% sodium azide. |
| 计算分子量 | 120 kDa |
| 参考文献 |
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SIRT1 mAb (Clone 2G1/F7) tested by Western blot. Nuclear extract (20 µg) of CMSL0 cells stained with SIRT1 mAb (Clone 2G1/F7) at a dilution of 1 µg/ml. |
