| 货号 | 39288 |
| 克隆类别 | 3A6-4C11 |
| 同种亚型 | IgG |
| 反应种属 | Hamster, Human, Monkey, Mouse, Rat |
| 来源宿主 | Mouse |
| 应用 | Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: WB: 0.5 - 2 µg/ml dilution ICC/IF: 2 - 5 µg/ml dilution Published Applications: see references Both lamin A and lamin C are recognized by this sera, with lamin A (the slower migrating band on Western blot) sometimes appearing as a doublet. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. For immunofluorescence experiments we recommend using paraformaldehyde (PFA) fixation. |
| 供应商 | Active Motif |
| 纯化方式 | Culture Supernatant |
| 免疫原 | This Lamin A/C antibody was raised against a recombinant protein corresponding to amino acids 430-545 of human Lamin A. |
| 背景 | Nuclearlamins are intermediate filament proteins that are the major structural component of the nuclear lamina on the inner surface of the nuclear envelope. Lamins AandLamins C are splice variants of the Lamin A gene. Lamin A/C(CDCD1,LMN1,EMD2) expression is a hallmark of embryonic stem cell differentiation. In addition to adding structural integrity to the nucleus, lamins contribute to the makeup of the nuclear matrix. Lamins also help organize interphase chromatin through interactions with several chromatin proteins, including histones and Lap2, such that alteration in lamin organization results in disruption of DNA replication, transcription and RNA processing. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | Cell culture supernatant containing mouse IgG, 30% glycerol, 0.035% sodium azide. |
| 计算分子量 | 75 kDa |
| 参考文献 |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Lamin A/C mAb (Clone 3A6-4C11) tested by immunofluorescence. Staining of HeLa cells with Lamin A/C mAb (Clone 3A6-4C11). Cells were fixed with 3.7% PFA for 15 minutes at room temperature, permeabilized with PBS + 0.5% Triton™ X-100 and stained with Lamin A/C mAb (2 µg/ml dilution) followed by 1:500 dilution Chromeo™ 546 Goat anti-mouse IgG (Cat No. 15033) (top panel) and DAPI (bottom panel). | |
Lamin A/C mAb (Clone 3A6-4C11) tested by Western blot. Whole-cell extract (20 µg) of HeLa cells stained with Lamin A/C mAb (Clone 3A6-4C11) at a dilution of 0.5 µg/ml. |
