Acid Sphingomyelinase Activity Colorimetric Assay Kit
货号：
K598-100 基本售价：
6310.5 元 规格：
100assays
产品信息
概述| 货号 | K598-100 |
| 描述 | Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphorylcholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger. There are five distinct types of SMases. Deficiency in Acid SMase (ASMase) leads to Niemann-Pick disease type A and B. BioVision’s Acid Sphingomyleinase Activity Assay Kit provides a simple and sensitive method for measuring ASMase enzymatic activity using colorimetry (OD 570 nm). In this assay, ASMase converts its substrate, sphingomyelin to phosphorylcholine and ceramide at pH 5.0; subsequently, phosphorylcholine is utilized in a series of reactions culminating in color formation from a highly specific probe. This high-throughput adaptable assay kit can detect ASMase activity as low as 3 mU/ml in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (570 nm)• Applications - Measurement of ASMase activity in various tissues/cell extracts• Screening of ASMase inhibitors |
| 样本类型以及上样量 | • Animal tissues: brain, heart, kidney, etc.• Cell culture: Adherent or suspension cells• Serum |
| 产品特色 | • Simple & Rapid Protocol• High-Throughput adaptable• This kit can detect ASMase activity as low as 3 mU/ml in a variety of samples. |
| 试剂盒组分 | • ASMase Assay Buffer I• ASMase Assay Buffer II• ASMase Substrate (Lyophilized)• ASMase Enzyme Mix I (Lyophilized)• ASMase Enzyme Mix II (Lyophilized)• Choline Standard (Lyophilized)• ASMase Positive Control (Lyophilized)• ASMase Probe (DMSO)• ASMase Enhancer |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
