Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit
货号：
K709-100 基本售价：
5947.5 元 规格：
100assays
产品信息
概述| 货号 | K709-100 |
| 描述 | Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food etc. • Species reactivity- Mammalian • |
| 产品特色 | • Simple procedure; takes ~ less than 40 minutes • Fast and convenient |
| 试剂盒组分 | • PK Assay Buffer • OxiRed™ Probe (in DMSO)• PK Enzyme Mix • PK Substrate Mix • PK Positive Control N/A• Pyruvate Standard (100 nmol/µl) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
