L-Carnitine Colorimetric/Fluorometric Assay Kit
货号：
K642-100 基本售价：
5826.5 元 规格：
100assays
产品信息
概述| 货号 | K642-100 |
| 描述 | Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where β-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active. BioVisions L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the Oxi-Red probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The detectio |
| 产品特色 | • Simple procedure; takes ~ 30 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of L-Carnitine levels |
| 试剂盒组分 | • Carnitine Assay Buffer• Carnitine Probe (in DMSO)• Carnitine Converting Enzyme • Carnitine Substrate Mix• Carnitine Development Mix • Carnitine Standard (10 µmol) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
