ADP Colorimetric Assay Kit II
货号：
K356-100 基本售价：
5584.5 元 规格：
100assays
产品信息
概述| 货号 | K356-100 |
| 描述 | ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. BioVision ADP Assay kit II is suitable for measuring ADP levels in samples that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP in the presence of ADP Enzyme Mix is converted to an intermediate, which reduces a colorless Probe to a colored product with strong absorbance at 450 nm. ADP Assay Kit II is simple, fast and high-throughput ready. It can detect less than 20 μM of ADP in samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Application - The kit can detect less than 20 μM of ADP in samples. |
| 样本类型以及上样量 | • Animal tissues - Liver, Muscle, Heart etc. • Cell culture - Adherent or suspension cells |
| 产品特色 | • Simple procedure; takes ~ 20 minutes • Convenient and sensitive • Kit contains all necessary reagents for accurate measurement of ADP levels |
| 试剂盒组分 | • ADP Assay Buffer • ADP Enzyme Mix (Lyophilized) • Developer (Lyophilized) • Probe (Lyophilized) • ADP Standard (Lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
