HAT Activity Fluorometric Assay Kit
货号：
K334-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K334-100 |
| 描述 | Histone Acetyltransferases (HATs) are enzymes that acetylate histone substrates resulting in important regulatory effects on chromatin structure and assembly, and gene transcription. Modifications of these proteins by HATs play an important role in the control of gene expression, and their dysregulation has been linked to cancer, neurodegeneration, and other diseases. BioVision’s HAT Activity Assay Kit utilizes Acetyl CoA and H3 histone peptide as substrates. In this assay, HAT enzyme catalyzes the transfer of acetyl groups from Acetyl-CoA to the histone peptide, thereby generating two products - acetylated peptide and CoA-SH. The CoA-SH reacts with the developer to generate a product that is detected fluorometrically at Ex/Em = 535/587 nm. The assay can detect HAT activity as low as 6 mU in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 535/587 nm)• Species reactivity - N/A• Applications:-Measurement of HAT activity in Nuclear Extracts- Measurement of HAT activity of purified enzyme preparations |
| 样本类型以及上样量 | • Nuclear extracts from cells and tissue• Recombinant enzyme |
| 产品特色 | • Simple, rapid & convenient• The assay can detect HAT Activity as low as 6 mU in a variety of samples |
| 试剂盒组分 | • HAT Assay Buffer• Acetyl CoA (Lyophilized) • H3 Peptide (Lyophilized)• Substrate Mix (Lyophilized)• Developer• PicoProbe™• CoA Standard (Lyophilized)• Positive Control (HeLa Nuclear Extract) |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
