HAT Activity Colorimetric Assay Kit
货号：
K332-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K332-100 |
| 描述 | Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. BioVision’s HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit utilizes active Nuclear Extract (NE) as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay. |
| 克隆号 | BA3R |
| 同种亚型 | Mouse IgG2b |
| 来源宿主 | Mouse |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (440 nm) • Sample type- Cell and Tissue lysates • Species reactivity- Mammalian • Applications- Detects HAT activity by colorimetric method in mammalian samples |
| 产品特色 | • Simple procedure • Fast and convenient • The detection can be continuous and suitable for kinetic studies |
| 试剂盒组分 | • 2X HAT Assay Buffer • HAT Substrate I • HAT Substrate II • NADH Generating Enzyme • HAT Reconstitution Buffer • Nuclear Extract 4 mg/ml |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
