Cytochrome Oxidase Activity Colorimetric Assay Kit
货号：
K287-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K287-100 |
| 描述 | Cytochrome c Oxidase (EC 1.9.3.1) or Complex IV is the fourth complex of the Electron Transport Chain located in the mitochondrial (or bacterial) membrane. It provides energy to the cell by coupling electron transport through the Cytochrome c chain with the process of oxidative phosphorylation. Complex IV contains 13 different subunits encoded by both mitochondrial DNA and nuclear DNA. It receives an electron from each of the four Cytochrome c molecules, and transfers it to one oxygen molecule, converting it into two molecules of water. In this process, it also binds to four proton molecules and translocates them across the membrane to establish electrochemical gradient, which is utilized for the synthesis of ATP. Cytochrome Oxidase Activity Assay Kit is simple, fast and high-throughput adaptable. This assay kit can be used for purified mitochondria or tissue extracts containing mitochondria. The activity of the enzyme is determined colorimetrically by following the oxidation of reduced Cytochrome c as an absorbance decrease at 550 nm. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 样本类型以及上样量 | • Purified mitochondria • Cells/Tissue extracts |
| 产品特色 | • Simple & Rapid • High-throughput adaptable • The kit provides unique formulation of buffers & reagents to measure cytochrome oxidase activity |
| 试剂盒组分 | • Cytochrome Oxidase Assay Buffer • Enzyme Dilution Buffer • 1 M DTT • Cytochrome c • 96-well Plate |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
