TEV Protease Activity Assay Kit (Fluorometric)
货号：
K842-100 基本售价：
4858.5 元 规格：
100assays
产品信息
概述| 货号 | K842-100 |
| 描述 | TEV Protease (EC: 3.4.22.44, Tobacco Etch Virus protease) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s TEV Protease Activity assay kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). This assay kit is simple, rapid and can detect TEV Protease activity as low as 50 ng/well in viral lysates and purified protein samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 490/560 nm)• Species reactivity - Viral lysates• Applications - Detection of TEV Protease activity in viral lysates - Determine activity of purified TEV Protease. |
| 样本类型以及上样量 | • Viral Lysates, purified protein |
| 产品特色 | • Simple, rapid assay• Can detect as low as 50 ng/well of TEV Protease activity• Fluorometric Detection |
| 试剂盒组分 | • TEV Protease Assay Buffer• TEV Protease Dilution Buffer• 1 M DTT• TEV Protease (Positive Control)• TEV Protease Substrate• 5-FAM Standard (100 µM in DMSO) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
