Isocitrate Colorimetric Assay Kit
货号：
K656-100 基本售价：
4751.0 元 规格：
100assays
产品信息
概述| 货号 | K656-100 |
| 描述 | Isocitric acid (HOOC-CHOH-CH (-COOH)-CH₂-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa. BioVisions Isocitrate Assay Kit provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In the assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. The Isocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 – 5 µg) of isocitrate. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Application- Isocitrate Assay Kit can detect 1 to 20 nmoles |
| 产品特色 | • Simple procedure; takes ~ 40 minutes • Fast and convenient • Kit contains all necessary reagents for accurate measurement of isocitrate levels |
| 试剂盒组分 | • Isocitrate Assay Buffer • Isocitrate Enzyme Mix • Substrate Mix • Isocitrate Standard (100 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
