Trypsin Activity Colorimetric Assay Kit
货号：
K771-100 基本售价：
4443.5 元 规格：
100assays
产品信息
概述| 货号 | K771-100 |
| 描述 | Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ=405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10-100 mU (p-NA unit) trypsin in various samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (405 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc. • Species reactivity- Mammalian • Application- The kit detects 10-100 mU (p-NA unit) tryp |
| 产品特色 | • Simple procedure; takes ~ 1-2 hours based on the levels of trypsin • Fast and convenient |
| 试剂盒组分 | • Trypsin Assay Buffer • Trypsin Substrate (in DMSO) • Positive Control (~2U, lyophilized) • p-NA Standard (2 mM) • Trypsin Inhibitor (TLCK, 20 mM) • Chymotrypsin Inhibitor (TPCK,10 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
