Sphingomyelinase Activity Colorimetric Assay Kit
货号：
K599-100 基本售价：
4443.5 元 规格：
100assays
产品信息
概述| 货号 | K599-100 |
| 描述 | Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation, and sterol homeostasis. There are five distinct types of SMases. BioVision’s SMase Activity Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using colorimetry (OD 570 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce an intermediate that reacts with a highly specific probe to generate color. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 1.25 mU/ml (~5 µU/mg tissue/cells) in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (OD 570 nm)• Applications:- Measurement of SMase activity in various tissues/cells extracts- Screening of SMase inhibitors |
| 样本类型以及上样量 | • Animal tissues: brain, heart, kidney, etc.• Cell culture: Adherent or suspension cells |
| 产品特色 | • Simple, rapid & convenient• The assay can detect sphingomyleinase activity as low as 1.25 mU/ml (~5 µU/mg tissue/cells) in a variety of samples |
| 试剂盒组分 | • SMase Assay Buffer• SMase Probe (DMSO)• SMase Substrate (Lyophilized)• SMase Enzyme Mix I (Lyophilized)• SMase Enzyme Mix II (Lyophilized)• Choline Standard (Lyophilized)• SMase Positive Control (Lyophilized)• SMase Extraction Detergent |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
