Nickel Colorimetric Assay Kit
货号：
K510-100 基本售价：
4443.5 元 规格：
100assays
产品信息
概述| 货号 | K510-100 |
| 描述 | Nickel is one of four ferromagnetic elements (symbol Ni,at. Num.28). Several enzymes depend on nickel for activity, including some ureases, carbon monoxide dehydrogenases (methane forming enzymes which reduce CO2 to CH4) and some hydrogenases which allow the production or removal of H2. Most of these activities are found in the archaebacteria. Nickel forms complexes with sulfhydryl compounds with significant absorbance in the UV/visible region in the presence of other ions. BioVision’s Nickel Assay kit provides a simple method of quantifying Ni2+ in a variety of samples. The assay takes advantage of reaction of Ni2+ with mercaptoethanol in borate buffer to form a complex with strong absorbance bands from ~300 to 600 nm. Fe2+ and Co+2 interfere with the assay, therefore extra steps (as described below) must be taken to subtract the interference in order to determine the correct Nickel concentration in mixed samples. Other ions tested (Mn2+, Cu2+, Zn2+) do not interfere with the assay, presumably no other ionic species are present in high enough concentration to interfere with the reaction. The assay is a simple method of quantifying Ni2+ in a variety of samples, which gives a linear range of 2 to 50 nmol Nickel containing less than 25 nmol Cobalt. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (300 to 600 nm) • Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- The assay is a simple method of quantify |
| 产品特色 | • Simple procedure; takes less than ~40 minutes • Fast and convenient |
| 试剂盒组分 | • Nickel Assay Buffer • Nickel Reagent • Nickel Chloride Standard (1.0 µmol) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
