TEV Protease Inhibitor Screening Kit (Fluorometric)
货号:
K843-100 基本售价:
4075.0 元 规格:
100assays
产品信息
概述货号 | K843-100 |
描述 | TEV Protease (EC: 3.4.22.44) is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). TEV Protease has high specificity and great stability and is active over a wide range of temperatures (4-37°C) with an optimal activity at 34°C. BioVision’s Inhibitor Screening Kit utilizes the ability of TEV Protease to cleave a synthetic Fluorescein-based peptide substrate to release fluorescein which can be easily quantified using a fluorometer or fluorescence microplate reader (Ex/Em = 490/560 nm). In the presence of a TEV Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of TEV Protease. |
同种亚型 | Rabbit IgG |
来源宿主 | Rabbit |
性能供应商 | Biovision |
检测类型 | Fluorescence (Ex/Em = 490/560 nm)• Species reactivity - N/A• Applications - Screening/studying/characterizing inhibitors of TEV Protease. |
样本类型以及上样量 | • Chemical libraries, small molecules |
产品特色 | • Simple, rapid assay• HTP Adaptable• Fluorometric Detection |
试剂盒组分 | • TEV Protease Assay Buffer• TEV Protease• TEV Protease Substrate• TEV Protease Inhibitor (2 mM) |
运输条件 | Blue Ice |
存放说明 | -20℃ |