Peroxidase Activity Colorimetric/Fluorometric Assay Kit
货号：
K772-100 基本售价：
3762.5 元 规格：
100assays
产品信息
概述| 货号 | K772-100 |
| 描述 | Peroxidases (EC number 1.11.1.x) are a large family of enzymes that typically catalyze a reaction of the form: ROOR + electron donor (2 e-) + 2H+ → ROH + ROH. For many of these enzymes the optimal substrate is hydrogen peroxide, but others are more active with organic hydroperoxides such as lipid peroxides. Peroxidases can contain a heme cofactor in their active sites, or alternately redox-active cysteine or selenocysteine residues. BioVision’s Peroxidase Assay Kit provides a convenient colorimetric and fluorometric means to measure the peroxidase activity in biological samples. In the presence of Peroxidase, the OxiRed Probe reacts with H2O2 in a 1:1 stoichiometry to produce the red-fluorescent oxidation product, resorufin. The resorufin is quantified by colorimetric (lmax = 570nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, direct, highly sensitive and high throughput-ready. The detection limit is 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (570 nm) or Fluorescence (Ex/Em=535/587)• Species reactivity- Mammalian • Application- The kit has a detection limit of 0.1 mU per well via colorimetric or 0.01 mU per well via fluorometric method, based on our unit definition. |
| 产品特色 | • Simple procedure • Fast and convenient • The assay is simple, direct, highly sensitive and high-throughput adaptable |
| 试剂盒组分 | • Assay Buffer• OxiRed™ Probe (in DMSO)• H2O2 Substrate (0.88 M)• HRP Positive Control |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
