| 货号 | 13296S |
| 反应种属 | All |
| 应用 | F |
| 目标/特异性 | The Mitochondrial Membrane Potential Assay Kit (II) is expected to detect the mitochondrial membrane potential in living cells cross all species. For the best result, a cell number titration is recommended when using a plate-reader and a 96-well plate. |
| 供应商 | CST |
| 背景 | Mitochondria are the main power house in cells and play important roles in processes such as steroid metabolism, calcium homeostasis, apoptosis and cellular proliferation. Mitochondrial membrane potential is a key indicator of its function and cell health (1,2). The dissipation of mitochondrial membrane potential is established as an early indicator for apoptosis (3). |
| 存放说明 | -20C |
| 参考文献 | 1 . Perry, S.W. et al. (2011) Biotechniques 50, 98-115. 2 . Nesti, C. et al. (2007) Biosci Rep 27, 165-71. 3 . Petit, P.X. et al. (1995) J Cell Biol 130, 157-67. 4 . OReilly, C.M. et al. (2003) Biophys J 85, 3350-7. |
Figure 2. Confocal immunofluorescent analysis of NIH/3T3 cells (2x105 cell/ml) seeded in a 96-well black plate with a clear bottom and incubated overnight. Cells were untreated or treated with CCCP (400 μM, 20 min) followed by labeling with TMRE (200 nM, 30 min). | |
Figure 3. HeLa cells (3x105 cell/ml) were treated with various concentrations of CCCP for 15 minutes prior to labeling with 200 nM TMRE. | |
Figure 1. Flow cytometric analysis of Jurkat cells, unlabeled (black) or labeled with 200 nM TMRE and treated with 0 μM CCCP (blue), 3.2 μM CCCP (green), or 80 μM CCCP (red). |
