CFSE Cell Division Assay Kit
货号：
10009853-100test 基本售价：
2030.0 元 规格：
100 tests
产品信息
概述| 货号 | 10009853-100test |
| 描述 | Cayman’s CFSE Cell Division Assay Kit provides an easy-to-use format for labeling and tracing cells through successive cell divisions. Carboxyfluorescein diacetate, succinimidyl ester (CFDA-SE) diffuses into cells, where the acetate groups are cleaved to yield a highly fluorescent derivative (CFSE) that is retained in the cell and can be detected by flow cytometry. Cell division results in sequential halving of fluorescence, and up to eight divisions can be monitored before the fluorescence is decreased to the background fluorescence of unstained cells.1,2 The ease of use and lack of cytotoxicity allows monitoring of cellular division over weeks either in vitroorin vivo. The kit contains sufficient reagents for labeling and analyzing up to 7.5x108 cells by flow cytometry. CFSE-labeled cells can be further stained with any fluorochrome compatible with fluorescein for use in flow cytometry. |
| 别名 | 5-Carboxyfluorescein NHS ester;5-Carboxyfluorescein-N-hydroxysuccinimide ester;5-Carboxyfluorescein N-Succinimidyl ester;5-FAM N-succinimidyl ester; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Parish, C.R.,Glidden, M.H.,Quah, B.J.C., et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Current Protocols in Immunology Supp. 84, 4.9.1-4.9.13 (2009).
2.Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. Journal of Immunological Methods 243, 147-154 (2000).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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