Luciferase Reporter Assay Kit
货号：
K801-200 基本售价：
1925.0 元 规格：
200assays
产品信息
概述| 货号 | K801-200 |
| 描述 | Firefly luciferase has been used as a sensitive reporter for studying gene regulation and function. Luciferase provides a 1000-fold increase in sensitivity in comparison to the standard chloramphenicol acetyltransferase (CAT) assay. Firefly luciferase catalyzes the oxidative carboxylation of luciferin, a reaction with the highest efficiency of any known bioluminescence reaction. The light emission from the reaction can be recorded using a luminometer. BioVision’s Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells. For measurement of expressed luciferase in vitro, luciferase is first extracted from transfected cells through cell lysis. Then CoA, ATP, Mg²ᶧ and buffer are added to the lysate (Inclusion of CoA yields a nearly constant light emission rather than typical flash Kinetics). The luminescent reaction is then triggered by an injection of luciferin, and the emitted light is recorded by a luminometer. By providing CoA, along with ATP, Mg²ᶧ in an optimized buffer solution, BioVision’s Luciferase Reporter Assay Kit ensure maximal sensitivity, consistent light output, as well as convenience and consistency when working with multiple samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Luminescence • Sample type- Cell and Tissue culture homogenates (eukaryotic cells) • Species reactivity- Mammalian • Application- This Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transf |
| 产品特色 | • Simple procedure; takes ~ 20-30 minutes • Fast and convenient • The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples. |
| 试剂盒组分 | • Substrate A • Substrate B • Cell Lysis Buffer |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
