| 货号 | 5562S |
| 供应商 | CST |
| 背景 | Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (2-4). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25a and bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (5-8). In this assay, the PTMScan® Ubiquitin Remnant Motif (K-ε-GG) Antibody Bead Conjugate, a proprietary ubiquitin branch (“K-ε-GG”) antibody with specificity for a di-glycine tag that is the remnant of ubiqui- tin left on protein substrates after trypsin digestion, is used to enrich ubiquitinated peptides from trypsin digested cell samples. This enrichment is followed by LC-MS/MS analy- sis for quantitative profiles of hundreds to over a thousand non-redundant ubiquitinated sequences. |
| 存放说明 | -20C |
| 参考文献 | 1 . Ciechanover, A. (1998) EMBO J 17, 7151-60. 2 . Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7. 3 . Hochstrasser, M. (2000) Science 289, 563-4. 4 . Bernardi, R. et al. (2000) Oncogene 19, 2447-54. 5 . Aberle, H. et al. (1997) EMBO J 16, 3797-804. 6 . Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3. 7 . Rush, J. et al. (2005) Nat Biotechnol 23, 94-101. 8 . Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21. |
Motif analysis was done using all ubiquitin remnant peptides in a ubiquitin remnant motif antibody PTMScan® study. Cellular tryptic peptides from HCT116 cells. untreated or treated with MG132, were immunoprecipitated with ubiquitin remnant motif antibody K-ε-GG and analyzed by OrbiTrap MS. The study gave 599 non-redundant sites. The motif logo shows that the K-ε-GG antibody is a general motif antibody that recognizes the K-ε-GG motif and does not have other amino acid preferences. 使用全部泛素残余抗体进行的PTMScan研究中发现的泛素残余肽段进行模体分析。HCT116细胞的细胞胰蛋白酶肽，未处理或MG132处理，与泛素残余模体抗体K-ε-GG进行免疫共沉淀，然后通过OrbiTrap MS分析。此研究给出599个非冗余位点。模体标识图显示K-ε-GG抗体是一个识别K-ε-GG模体的全模体抗体，对其它氨基酸没有偏好。 |
