| 货号 | D3400 |
| 描述 | The Quantikine Human IL-34 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-34 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-34 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-34. Results obtained using natural human IL-34 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-34. |
| 别名 | C16orf77; | 全称 | Interleukin 34 |
| 反应种属 | Human |
| 目标/特异性 | Natural and recombinant human IL-34 |
| 供应商 | R&D Systems |
| 检测类型 | Solid Phase Sandwich ELISA |
| Entrez Gene IDs | 146433 (Human); 76527 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-34 is associated with the presence and severity of renal dysfunction and coronary artery disease in patients with heart failure | |
| 生物活性 | < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested. |
| 背景 | Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level. IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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