Vitamin D3 (human) ELISA kit
货号：
K4806-100 基本售价：
6734.0 元 规格：
100assays
产品信息
概述| 货号 | K4806-100 |
| 描述 | Vitamin D3 (Cholecalciferol) is found in human epidermis and dermis. BioVision’s human Vit. D3 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. Human Vit. D3 antibody is coated on a 96-well plate. Standards and test samples are added to the wells and Vit. D3 present in a sample is bound by the immobilized antibody. An HRP-conjugate reagent is added subsequently. After washing away the unbound antibody/HRP conjugates, HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human Vit. D3 captured onto the plate. This ELISA kit shows no species cross-reactivity. Detection Range: 20 – 800 µg/L. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma (EDTA/Citrate)• Urine• Cell culture medium, Tissue homogenates and cell lysates |
| 产品特色 | • Easy, convenient and time-saving method to assay for human vitamin D3. • Detection Range:200 – 800 ug/L. • This ELISA kit shows no species cross-reactivity. |
| 试剂盒组分 | • 96 wells coated with anti-human Vit. D3 antibody, 1 Microplate • Human Vit. D3 standard (0.9 ng/µl)• HRP-conjugate reagent• Standard Diluent• Sample diluent• Chromogen Solution A• Chromogen Solution B• Stop Solution• Wash Solution (30x stock)• Sealed Bag• Plate cover |
| 运输条件 | Blue Ice |
| 存放说明 | +4℃ |
