| 货号 | 7689C |
| 描述 | The PathScan® Total Fatty Acid Synthase Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of fatty acid synthase protein (FASN). A FASN Rabbit Antibody has been coated onto the microwells. After incubation with cell lysates, FASN is captured by the coated antibody. Following extensive washing, a biotinylated FASN Rabbit Detection Antibody is added to detect the captured FASN protein. HRP-linked streptavidin is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of FASN. Antibodies in kit are custom formulations specific to kit. |
| 反应种属 | Human/Mouse/Monkey |
| 应用 | ELISA |
| 目标/特异性 | This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FASN is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FASN is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1). Recently, increased expression of FASN has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FASN are directly related to the size of breast tumors (2). Studies also showed that FASN is highly expressed in lung and prostate cancers and that FASN expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FASN is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FASN as a potential target for the diagnosis and treatment of cancer as well as metabolic syndrome (6,7). |
| 存放说明 | 4C |
| 参考文献 | Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33. Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40. Kawamura, T. et al. (2005) Pathobiology 72, 233-240. Shah, U.S. et al. (2006) Hum Pathol 37, 401-409. Kuhajda, F.P. (2000) Nutrition 16, 202-8. Tian, W.X. (2006) Curr Med Chem 13, 967-977. Kusunoki, J. et al. (2006) Endocrine 29, 91-100. |
Figure 1. Treatment of NIH/3T3 cells with LY294002 decreases expression of FASN as detected by the PathScan® Total Fatty Acid Synthase Sandwich ELISA Kit #7689. NIH/3T3 cells (60-70% confluent) were treated with LY294002 #9901 (16 μM, 48 hr). The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using Fatty Acid Synthase (C20G5) Rabbit mAb #3180 is shown in the bottom figure. 图1. 使用LY294002处理NIH/3T3细胞使FASN表达减少，使用Total Fatty Acid Synthase Sandwich ELISA Kit #7689进行检测。NIH/3T3细胞（60-70%汇总）经LY294002 #9901（16ul，48小时）处理。上图为在450nm处的吸光度读数，相应的，下图显示使用Fatty Acid Synthase (C20G5) Rabbit mAb兔单抗 #3180的Western blot结果。 | |
Figure 2. The relationship between the protein concentration of lysates from control NIH/3T3 cells or LY294002-treated NIH/3T3 cells and the absorbance at 450 nm is shown. NIH/3T3 cells (60-70% confluent) were treated with LY294002 #9901 (16 μM, 48 hr) and then lysed. 图2. 显示对照和LY294002处理的NIH/3T3细胞裂解液中的蛋白浓度与试剂盒光学浓度读数的关系。NIH/3T3细胞（60-70%汇总）经LY294002 #9901（16ul，48小时）处理后裂解。 |
