PathScan® Total PTEN Sandwich ELISA Kit【科瑞奥博】

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PathScan® Total PTEN Sandwich ELISA Kit

货号： 7882C 基本售价： 6346.0 元规格： 1Kit

产品信息

概述
货号	7882C
描述	The PathScan® Total PTEN Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenously expressed PTEN. A PTEN rabbit antibody has been coated onto the microwells. After incubation with cell lysates, PTEN is captured by the coated antibody. Following extensive washing, a PTEN mouse detection antibody is added to detect the captured PTEN. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate (TMB) is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of PTEN.
反应种属	Human/Mouse/Rat/Monkey
应用	ELISA
目标/特异性	CSTs PathScan® Total PTEN Sandwich ELISA Kit #7882 detects endogenous levels of PTEN. As shown in Figure 1, using the Total PTEN Sandwich ELISA Kit #7882, a high level of PTEN is detected in a variety of PTEN positive cell lines but not in the PTEN negative cell line PC-3. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

性能
供应商	CST
背景	PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10).
存放说明	4C
参考文献	Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. Funamoto, S. et al. (2002) Cell 109, 611-23. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.

参考图片

Figure 1. PTEN is detected by PathScan® Total PTEN Sandwich ELISA Kit #7882. Lysates were prepared from various cell lines and analyzed for PTEN using PathScan® Total PTEN Sandwich ELISA Kit #7882 or by western blotting. Absorbance readings at 450 nm are shown in the top figure while the corresponding western blot using PTEN Antibody #9559 is shown in the bottom figure.

Figure 1，采用PathScan ® Total PTEN Sandwich ELISA Kit #7882检测PTEN ，裂解液从不同细胞系中提取，分析用试剂盒为PathScan ® Total PTEN Sandwich ELISA Kit #7882 或western blotting。在450nm处读取吸光度值（上图），相对应western blot采用抗体为PTEN Antibody #9559 ，见下图。

Figure 2. The relationship between the protein concentration of the lysate from PTEN positive and PTEN negative cell lines and the absorbance at 450 nm is shown.

Firure2 PTEN阴性和阳性细胞系中蛋白浓度与450nm处吸光度值的关系图。