| 货号 | 14507C |
| 反应种属 | Human/Monkey |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Ki-67 Sandwich ELISA Kit detects endogenous levels of Ki-67 protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4). |
| 存放说明 | 4C |
| 参考文献 | Gerdes, J. et al. (1983) Int J Cancer 31, 13-20. Weigel, M.T. and Dowsett, M. (2010) Endocr Relat Cancer 17, R245-62. Jones, R.L. et al. (2009) Breast Cancer Res Treat 116, 53-68. Yerushalmi, R. et al. (2010) Lancet Oncol 11, 174-83. |
Figure 1. Ki-67 protein is detected at varying levels from multiple cell lines using the PathScan® Total Ki-67 Sandwich ELISA Kit #14507. The absorbance readings at 450 nm are shown in the top figure, while corresponding western blots using Ki-67 (D3B5) Rabbit mAb #9129 are shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from HeLa cells and the absorbance at 450 nm as detected by the PathScan® Total Ki-67 Sandwich ELISA Kit #14507 is shown. HeLa cells (85% confluence) were harvested and then lysed. |
