| 货号 | 13907C |
| 反应种属 | Human/Rat |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total BACE Sandwich ELISA Kit detects endogenous and transfected levels of BACE protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | β-secretase (BACE) is an aspartic acid proteinase that catalyses the initial step in APP processing by cleaving and releasing a soluble, extracellular APP-β (sAPPβ) ectodomain and generating a membrane-bound, carboxy-terminal fragment consisting of 99 amino acids (CTF99). Additional processing of CTF99 by γ-secretase generates the amyloid β-peptide (Aβ) that forms aggregates in the brains of Alzheimer disease (AD) patients. BACE is an attractive target for inhibitors in AD therapy since it catalyses the first and rate limiting step in amyloidogenic APP processing (1). Pro-BACE-1 is synthesized in the ER before it is transported to the trans-Golgi network to undergo maturation (2). BACE-1 functions as a dimer and is phosphorylated at Ser498 by casein kinase 1, which is necessary for recycling back to the membrane from the early endosome (3,4). |
| 存放说明 | 4C |
| 参考文献 | Hunt, C.E. and Turner, A.J. (2009) FEBS J 276, 1845-59. Benjannet, S. et al. (2001) J Biol Chem 276, 10879-87. Walter, J. et al. (2001) J Biol Chem 276, 14634-41. He, X. et al. (2005) J Biol Chem 280, 11696-703. |
Figure 1: BACE protein was detected from rat brain lysate and HeLa cell lysates, untransfected (-) or transfected with a construct expressing BACE protein (+), using the PathScan® Total BACE Sandwich ELISA Kit #13907. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using BACE (D10E5) Rabbit mAb #5606 is shown in the lower figure. | |
Figure 2: The relationship between protein concentration of lysates from HeLa cells, untransfected or transfected with a construct expressing BACE protein, and the absorbance at 450 nm as detected by the PathScan® Total BACE Sandwich ELISA Kit is shown. |
