| 货号 | 14520C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total B7-H4 Sandwich ELISA Kit detects endogenous levels of B7-H4 protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | B7 homolog 4 (B7-H4, VTCN1) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses (1-3). B7-H4 protein contains two extracellular Ig-like V-type domains, a transmembrane domain, and a short, two amino acid intracellular domain (3). The B7-H4 protein is shown to inhibit T cell activation, proliferation, and cytokine production (1,4,5). Although B7-H4 mRNA is widely expressed, B7-H4 protein is restricted to antigen presenting cells and B cells (1). The B7-H4 protein is also found in several tumor types, including ovarian cancer and breast cancer (6). Research studies indicate that B7-H4 protein is present on the surface of ovarian tumor cells, and that targeted inhibition of B7-H4 using recombinant antibodies restores T cell activation pathways. These studies suggest some potential therapeutic value in blocking B7-H4 function and restoring T cell function in cancer patients (7,8). |
| 存放说明 | 4C |
| 参考文献 | Sica, G.L. et al. (2003) Immunity 18, 849-61. Prasad, D.V. et al. (2003) Immunity 18, 863-73. Zang, X. et al. (2003) Proc Natl Acad Sci U S A 100, 10388-92. He, C. et al. (2011) Clin Dev Immunol 2011, 695834. Yi, K.H. and Chen, L. (2009) Immunol Rev 229, 145-51. Salceda, S. et al. (2005) Exp Cell Res 306, 128-41. Dangaj, D. and Scholler, N. (2013) Oncoimmunology 2, e25913. Dangaj, D. et al. (2013) Cancer Res 73, 4820-9. |
Figure 1. B7-H4 protein is expressed in SK-BR-3 cells but absent in H1299 cells, as detected using the PathScan® Total B7-H4 Sandwich ELISA Kit #14520. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blot using B7-H4 (D1M8I) XP® Rabbit mAb #14572 is shown in the bottom figure. | |
Figure 2. The relationship between protein concentration of lysates from SK-BR-3 cells and the absorbance at 450 nm as detected by the PathScan® Total B7-H4 Sandwich ELISA Kit #14520 is shown. SK-BR-3 cells (85% confluence) were harvested and then lysed. |
