| 货号 | 12850C |
| 描述 | The PathScan® Total Androgen Receptor Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total androgen receptor protein. An Androgen Receptor Rabbit mAb has been coated onto the microwells. After incubation with cell lysates, androgen receptor protein is captured by the coated antibody. Following extensive washing, an Androgen Receptor Mouse Detection mAb is added to detect the captured androgen receptor protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of absorbance for the developed color is proportional to the quantity of total androgen receptor protein. Antibodies in kit are custom formulations specific to kit.PathScan® Total Androgen Receptor Sandwich ELISA Kit是一个固相夹心酶联免疫吸附试验（ELISA），能够检测内源性水平的总雄激素受体蛋白。微孔已经包被Androgen Receptor Rabbit mAb。细胞裂解物孵育后，雄激素受体蛋白由包被的抗体捕获。充分洗涤后，加入Androgen Receptor Mouse Detection mAb检测捕获的雄激素受体蛋白。然后用抗小鼠IgG，HRP标记的抗体识别结合的检测抗体。加入HRP底物TMB进行显色。显色吸光度的大小与总雄激素受体蛋白量成正比。该试剂盒中的抗体是针对试剂盒定制的。 |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Total Androgen Receptor Sandwich ELISA Kit recognizes endogenous levels of androgen receptor protein as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding (1). This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer (2,3).雄激素受体（AR）是锌指转录因子，属于核受体超家族，通过磷酸化和配体结合的二聚化被激活（1）。这促进了细胞核定位和雄激素靶基因中雄激素与应答元件的结合。 AR在男性发育和前列腺癌发展的几个阶段起着至关重要的作用（2,3）。 |
| 存放说明 | 4C |
| 参考文献 | Li, J. and Al-Azzawi, F. (2009) Maturitas 63, 142-8. Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142. Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146. |
Figure 1. Androgen receptor protein from human (HeLa, MCF7, T-47D, LNCaP, or U-87 MG) cells was detected using PathScan® Total Androgen Receptor Sandwich ELISA Kit. However, this kit has no detection in human (Jurkat, K-562, or PC-3), monkey (COS-7), hamster (CHO), mouse (NIH/3T3, C2C12), or rat (C6, PC-12) cells. The absorbance readings at 450 nm are shown in the top figure, while corresponding western blots using Androgen Receptor (D6F11) XP® Rabbit mAb #5153 are shown in the bottom figure.图1. 用PathScan® Total Androgen Receptor Sandwich ELISA Kit检测人细胞(HeLa, MCF7, T-47D, LNCaP或U-87 MG)雄激素受体蛋白。但是，该试剂盒不能检测人细胞（Jurkat, K-562或PC-3）、猴子细胞（COS-7）、仓鼠细胞（CHO）、小鼠细胞（NIH/3T3, C2C12）或大鼠细胞(C6, PC-12)。上图显示在450 nm处的吸光度值，下图显示与其对应的western blots结果，使用的抗体为Androgen Receptor (D6F11) XP® Rabbit mAb #5153. | |
Figure 2. The relationship between protein concentration of lysates from LNCaP cells and the absorbance at 450 nm is shown. LNCaP cells (85% confluence) were harvested and then lysed.图2. 如图所示，LNCaP细胞裂解物蛋白浓度与450 nm处吸光度值的关系。LNCaP细胞长至85%汇合后收集，并随后裂解。 |
