| 货号 | 13491C |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-M-CSF Receptor (panTyr) Sandwich ELISA Kit recognizes endogenous levels of tyrosine-phosphorylated M-CSF receptor protein in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
Figure 1: Treatment of NKM-1 cells with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 stimulates tyrosine phosphorylation of M-CSF receptor protein, as detected by PathScan® Phospho-M-CSF Receptor (panTyr) Sandwich ELISA Kit, but does not affect the level of total M-CSF receptor detected by PathScan® Total M-CSF Receptor Sandwich ELISA Kit #13032. The absorbance readings at 450 nm are shown in the top figure while corresponding western blots using M-CSF Receptor Antibody #3152 (left panel) and Phospho-M-CSF Receptor (Tyr699) (D10B11) Rabbit mAb #12251 (right panel) are shown in the bottom figure. | |
Figure 2: The relationship between protein concentration of lysates from untreated and hM-CSF-treated NKM-1 cells and the absorbance at 450 nm as detected by the PathScan® Phospho-M-CSF Receptor (panTyr) Sandwich ELISA Kit is shown. Unstarved NKM-1 cells (0.5-1.0 x 106) were treated with Human Macrophage Colony Stimulating Factor (hM-CSF) #8929 (50 ng/ml) for 2-5 min at 37°C and then lysed. |
