| 货号 | 7021C |
| 描述 | The PathScan® Phospho-FLT3 (Tyr591) Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of phospho-FLT3 (Tyr591) protein with a chemiluminescent readout. Chemiluminescent ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using a smaller sample size. A FLT3 Mouse mAb has been coated on the microwells. After incubation with cell lysates, FLT3 (phospho or nonphospho) protein is captured by the coated antibody. Following extensive washing, a phospho-FLT3 (Tyr 591) Rabbit Antibody is added to detect the captured phospho-FLT3 protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of phospho-FLT3 (Tyr591) protein. |
| 反应种属 | Human |
| 应用 | ELISA |
| 目标/特异性 | PathScan® Phospho-FLT3 (Tyr591) Chemiluminescent Sandwich ELISA Kit #7021 detects endogenous levels of phospho-FLT3 (Tyr591) in human cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. |
| 供应商 | CST |
| 背景 | FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). |
| 存放说明 | 4C |
| 参考文献 | Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48. Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30. Namikawa, R. et al. (1996) Stem Cells 14, 388-95. Beslu, N. et al. (1996) J Biol Chem 271, 20075-81. Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9. Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80. Mizuki, M. et al. (2000) Blood 96, 3907-14. |
Relationship between protein concentration of lysates from phospho or nonphospho SEM cells and immediate light generation with chemiluminescent substrate is shown. SEM cells in exponential growth (106 cells/ml) were lysed with or without the addition of phosphatase inhibitor to the lysis buffer (phospho or nonphospho lysate). The graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.磷酸化的和未磷酸化的SEM细胞的裂解中蛋白含量和化学发光底物发光值之间的关系。指数生长期的SEM cells (106 cells/ml)用加或不加磷酸酶抑制剂的裂解液进行裂解。插入的图表是阴影区域的放大图，表示在低目的蛋白浓度时的检测有很高的灵敏度和线性关系。 |
