| 货号 | NBP2-24683PEP |
| 克隆类别 | Polyclonal |
| 反应种属 | Hu, Mu, Rt, PmSpecies Glossary |
| 来源宿主 | Rabbit |
| 应用 | WB, ICC/IF, IHC, IHC-P |
| 供应商 | Novus |
| 免疫原 | A portion of amino acids 310-360 of human STING was used as the immunogen. |
| 背景 | STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA from bacteria/viruses or the self (intrinsic) and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING has been suggested to interact with DDX58/RIG-I, MAVS, SSR2, RNF5, TRIM56, TBK1, IFIT1 and IFIT2. It generally localize to the cytoplasm, and membranes of cell, ER and mitochondria but in response to DNA stimulation, it translocate to the perinuclear region for interaction with TBK1 kinase. The latter phosphorylates STING at Ser-358 residue which results in its activation. STING executes its role by spotting and binding cyclic di-GMP / c-di-GMP and cyclic GMP-AMP /cGAMP which follows the activation of NF-kappa B and IRF3 transcriptional signaling pathways leading to the induction of Type I interferon response. STING has also been suggested to involve in cell death signaling pathways through its association with MHC-II and the activation of ERK pathway. Besides immune response to bacterial/viral pathogens, innate immune gene transcription has been of great importance in the elucidation of the causes of auto-inflammatory disease involving the sensing of self-DNA and the generation of effective antitumor adaptive immunity. STING regulated innate signaling has offered critical insights and new opportunities into the development of novel immunization regimes and therapeutics for treatment of infection, autoimmune disorders, inflammation and cancer. |
| 运输条件 | Dry Ice |
| 存放说明 | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| 存储溶液 | PBS |
| 浓度 | 1.0 mg/ml |
| 纯度 | Protein A purified |
Western Blot: STING/TMEM173 Antibody [NBP2-24683] - Whole cell protein from THP1- cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-STING/TMEM173 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Arrowheads indicate protein monomer and possible dimer. | |
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Analysis of STING protein in formalin-fixed paraffin-embedded mouse lung tissue section using STING antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells. | |
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - IHC analysis of STING in formalin-fixed paraffin-embedded human colon cancer tissue section using STING antibody at 1:100 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated very weak cytoplasmic staining in columner epithelial cells with a very strong signal in the secretory/goblet cells. | |
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody [NBP2-24683] - Analysis of STING protein in formalin-fixed paraffin-embedded mouse lung tissue section using STING antibody at 1:150 dilution with detection employing HRP-conjugated secondary antibody. The signal was developed using DAB reagent and the nuclei were counterstained with hematoxylin. The antibody generated mainly a cytoplasmic staining in the bronchiolar and alveolar epithelial cells. |
