| 货号 | 2915T |
| 描述 | The Wnt Signaling Antibody Sampler Kit provides an economical means of detecting integral proteins within the Wnt signaling pathway. The kit contains enough primary and secondary antibody to perform four Western blots with each. |
| 目标/特异性 | Wnt3a (C64F2) Rabbit mAb #2721 detects transfected Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells. Phospho-LRP6 (Ser1490) Antibody #2568 detects endogenous levels of LRP6 protein when phosphorylated at Ser1490. The remaining antibodies in the Wnt Signaling Sampler Kit detect endogenous levels of their respective total proteins. |
| 供应商 | CST |
| 背景 | The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (2). Wnt members bind to the Frizzled family of proteins and activate several signaling pathways (3). The canonical Wnt/β-catenin pathway requires a coreceptor from proteins belonging to the low-density lipoprotein receptor (LDLR) family, such as LRP5 and LRP6 (4). Upon stimulation with Wnt, LRP6 is phosphorylated by kinases such as GSK-3 and CK1 and subsequently recruits Axin to the membrane (5-7). In the absence of Wnt stimulation, the multidomain scaffold proteins Axin1 and Axin2 negatively regulate Wnt signaling. Axin1 complexes with APC, GSK-3β, Dvl and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin (8-9). The Disheveled (Dsh) proteins (Dvl1, Dvl2 and Dvl3) inhibit glycogen synthase kinase-3β, thereby promoting β-catenin stabilization (4,10). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dsh proteins and directing Dsh activity towards the planar cell polarity pathway (11-12). |
| 存放说明 | -20C |
| 参考文献 | 1 . Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. 2 . Luo, W. and Lin, S.C. Neurosignals 13, 99-113. 3 . Salahshor, S. and Woodgett, J.R. (2005) J. Clin. Pathol. 58, 225-236. 4 . Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37. 5 . Wharton, K.A. et al. (2001) Dev Biol 234, 93-106. 6 . Tamai, K. et al. (2000) Nature 407, 530-535. 7 . Yan, D. et al. (2001) Proc Natl Acad Sci U S A 98, 3802-7. 8 . Giles, R.H. et al. (2003) Biochim Biophys Acta 1653, 1-24. 9 . Logan, C.Y. and Nusse, R. (2004) Annu Rev Cell Dev Biol 20, 781-810. 10 . Kohn, A.D. and Moon, R.T. Cell Calcium 38, 439-46. 11 . Zeng, X. et al. (2005) Nature 438, 873-877. 12 . Davidson, G. et al. (2005) Nature 438, 867-872. |
Western blot analysis of extracts from Hep G2 and HeLa cells using LRP6 (C47E12) Rabbit mAb. | |
Western blot analysis of extracts from Hep G2 and HeLa cells using LRP6 (C47E12) Rabbit mAb #3395. | |
Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb #2073.使用Naked1 (C67C4) 兔单抗#2073对多种细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb #2087.使用Axin1 (C76H11) 兔单抗#2087对多种细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from HepG2 and SW620 cells using Naked1 (C30F10) Rabbit mAb #2201.使用Naked1 (C30F10) 兔单抗#2201对HepG2和SW620细胞全细胞提取物进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Dvl3 Antibody #3218.使用Dvl3抗体#3218对多种细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from various cell lines using Dvl2 (30D2) Rabbit mAb #3224.使用Dvl2 (30D2) 兔单抗#3224对多种细胞全细胞提取物进行western blot分析。 | |
Western blot analysis of total cell lysates from HeLa cells untreated, treated 5 hours with conditioned medium (CM) from L cells, or treated 5 hours with conditioned medium from L cells overexpressing Wnt3a, using Phospho-LRP6 (Ser1490) Antibody #2568.使用 Phospho-LRP6 (Ser1490)抗体#2568对未处理或用L细胞条件培养基或过表达Wnt3a的L细胞条件培养基处理5小时的HeLa细胞全细胞提取物进行进行western blot分析。 | |
Western blot analysis of total cell lysates from HeLa, LN18, PANC1 cells and conditioned medium (CM) from HeLa cells, using Wnt5a/b (C27E8) Rabbit mAb #2530.使用Wnt5a/b (C27E8)兔单抗#2530对HeLa，LN18，PANC1全细胞提取物和HeLa细胞条件培养基提取物进行western blot分析。 | |
Western blot analysis of total cell lysates and conditioned medium (CM) from L cells overexpressing mouse Wnt3a, compared with parental L cells, using Wnt3a (C64F2) Rabbit mAb #2721.使用Wnt3a (C64F2) 兔单抗#2721对过表达小鼠wnt3a的L细胞及对照组亲本L细胞的全细胞提取物和条件培养基进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using Naked2 (C67C4) Rabbit mAb. | |
Western blot analysis of total cell lysates and conditioned medium from L cells overexpressing mouse Wnt3a, compared with parental L cells, using Wnt3a (C64F2) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using Axin1 (C76H11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Axin1 (C76H11) Rabbit mAb. | |
Western blot analysis of total cell lysates from HepG2 and SW620 cells using Naked1 (C30F10) Rabbit mAb. |
