USP Antibody Sampler Kit【科瑞奥博】

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USP Antibody Sampler Kit

货号： 12894T 基本售价： 7504.0 元规格： -

产品信息

概述
货号	12894T
目标/特异性	Each antibody in the USP Antibody Sampler Kit recognizes endogenous levels of its respective target protein, except for USP28, which detects transfected levels of its target protein. USP2 Antibody cross-reacts with all known USP2 splice variants but does not cross-react with USP21. USP9X Antibody may also cross-react with USP9Y. Based on sequence alignment, USP14 Antibody is predicted to cross-react with both isoform a and isoform b of USP14. The doublet band detected by western blot for USP18 (D4E7) Rabbit mAb represents full-length (39 kDa) and amino-terminal deleted derivative of USP18 (31).

性能
供应商	CST
背景	Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). The ubiquitin-specific protease (USP) subfamily is one of five distinct groups of DUB enzymes. Ubiquitin-specific-processing protease 1 (USP1) is regulated in a cell cycle dependent manner by both transcriptional and ubiquitin-proteasomal mechanisms (3). Nuclear USP1 localizes to chromatin where it deubiquitinates monoubiquitinated FANCD2 and plays an important role in DNA damage repair and Chk1 protein stability (3,4). Ubiquitin-specific-processing protease 2 (USP2) contains C19 peptidase activity and is involved in ubiquitin recycling and disassembly of polymeric ubiquitin and ubiquitin-like protein complexes (5). USP2 is a putative oncoprotein that is highly over expressed in prostate cancer and drives tumor growth by binding and stabilizing fatty acid synthase through deubiquitination (6,7).
存放说明	-20C
参考文献	1 . Nijman, S.M. et al. (2005) Cell 123, 773-86. 2 . Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3 . Nijman, S.M. et al. (2005) Mol Cell 17, 331-9. 4 . Guervilly, J.H. et al. (2011) Hum Mol Genet 20, 2171-81. 5 . Wilkinson, K.D. (1997) FASEB J 11, 1245-56. 6 . Graner, E. et al. (2004) Cancer Cell 5, 253-61. 7 . Priolo, C. et al. (2006) Cancer Res 66, 8625-32. 8 . Li, M. et al. (2002) Nature 416, 648-53. 9 . Malakhov, M.P. et al. (2002) J. Biol. Chem. 277, 9976-9981. 10 . Brooks, C.L. et al. (2007) Oncogene 26, 7262-6. 11 . van der Horst, A. et al. (2006) Nat Cell Biol 8, 1064-73. 12 . Oh, Y.M. et al. (2007) Biochem Biophys Res Commun 357, 615-9. 13 . Naviglio, S. et al. (1998) EMBO J 17, 3241-50. 14 . Niendorf, S. et al. (2007) Mol Cell Biol 27, 5029-39. 15 . Murray, R.Z. et al. (2004) Mol Biol Cell 15, 1591-9. 16 . Théard, D. et al. (2010) EMBO J 29, 1499-509. 17 . Soncini, C. et al. (2001) Oncogene 20, 3869-79. 18 . Yuan, J. et al. (2010) Cell 140, 384-96. 19 . Lee, B.H. et al. (2010) Nature 467, 179-84. 20 . Koulich, E. et al. (2008) Mol Biol Cell 19, 1072-82. 21 . Hanna, J. et al. (2006) Cell 127, 99-111. 22 . Thrower, J.S. et al. (2000) EMBO J 19, 94-102. 23 . Rempel, L.A. et al. (2007) Reprod Biol Endocrinol 5, 13. 24 . Ritchie, K.J. et al. (2002) Genes Dev 16, 2207-12. 25 . Zhang, D. et al. (2006) Cell 126, 529-42. 26 . Popov, N. et al. (2007) Nat Cell Biol 9, 765-74.

参考图片

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from COS cells, untransfected or transfected with mouse USP28, using USP28 Antibody.

Western blot analysis of extracts from THP-1 cells, untreated or LPS-treated (1 µg/ml, 24 hours), using USP18 (D4E7) Rabbit mAb.

Confocal immunofluorescent analysis of MCF7 cells using HAUSP (D17C6) XP^® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Western blot analysis of extracts from various cell lines using HAUSP (D17C6) XP^® Rabbit mAb.

Western blot analysis of extracts from various cell lines using USP9X Antibody.

Western blot analysis of extracts from HeLa, A20 and COS cells using USP1 (D37B4) Rabbit mAb.

Western blot analysis of extracts from various cell lines using USP2 Antibody.

Western blot analysis of extracts from COS-7 cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length transcript variant 1 of human USP2 (+), using USP2 Antibody.

Western blot analysis of extracts from various cell lines using USP8 Antibody.

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected (+) with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP8, using USP8 Antibody.

Western blot analysis of extracts from various cell lines using USP14 Antibody.

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a DDK-tagged cDNA expression construct encoding full-length isoform a of human USP14 (hUSP14-DDK, +), using USP14 Antibody.

Confocal immunofluorescent analysis of HCT 116 cells using USP10 (D7A5) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using USP10 (D7A5) Rabbit mAb.