| 货号 | 4753T |
| 描述 | The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or four Western blots with each antibody. |
| 目标/特异性 | Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components. |
| 供应商 | CST |
| 背景 | Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.一个蛋白质的亚细胞定位的了解可能揭示其在不同细胞进程中潜在角色。其中一点是能够确定一个标记物的亚细胞定位，该标记物是与该试剂盒中细胞器特异性抗体之一发生共定位。当这些抗体作为免疫荧光的强大工具，对于细胞裂解它们也可能被使用在免疫印迹（western blot）实验内参照。Application References |
| 存放说明 | -20C |
Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 uM, overnight; right) using LC3B (D11) XP® Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Flow cytometric analysis of human peripheral blood lymphocytes using Histone H3 (D1H2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. | |
Western blot analysis of extracts from various cell lines, untreated (-) or treated overnight with chloroquine (50 μM) (+), using LC3B (D11) XP® Rabbit mAb (upper) or LC3B Antibody #2775 (lower). | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® LC3B siRNA I #6212 (+) or SignalSilence® LC3B siRNA II #6213 (+), using LC3B (D11) XP® Rabbit mAb #3868 and α-Tubulin (11H10) Rabbit mAb #2125. The LC3B (D11) XP® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Histone H3 (D1H2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP® Rabbit mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red). | |
Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded HeLa cell pellets, control (left) or chloroquine-treated (right), using LC3B (D11) XP® Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human astrocytoma using LC3B (D11) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using Rab5 (C8B1) Rabbit mAb. | |
Flow cytometric analysis of HeLa cells using LC3B (D11) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Confocal immunofluorescent analysis of HeLa cells using Rab5 (C8B1) Rabbit mAb (green). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb #4850 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ™ (fluorescent DNA dye).使用COX IV (3E11) Rabbit mAb #4850（绿色）标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝（红色）。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb #2639 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Fibrillarin (C13C3) Rabbit mAb #2639（绿色）标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝（红色）。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Confocal immunofluorescent analysis of HeLa cells using Rab5 (C8B1) Rabbit mAb #3547 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Rab5 (C8B1) Rabbit mAb #3547（绿色）标记，共聚焦免疫荧光分析HeLa细胞。蓝色= DRAQ5® #4084 (DNA荧光染料)。 |
