| 货号 | 4763T |
| 描述 | The Translation Initiation Complex Antibody Sampler Kit contains reagents to investigate the initiation of translation within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody. |
| 目标/特异性 | Each antibody in the Translation Initiation Complex Antibody Sampler Kit detects endogenous levels of its target protein. |
| 供应商 | CST |
| 背景 | A variety of factors contribute to the important biological event of translation initiation. The Eukaryotic initiation Factor 4E (eIF4E) complex of translation initiation factors binds to the 5 m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5 mRNA untranslated region to mediate ribosome binding (2,3). EIF4E binds to the mRNA cap structure to mediate the initiation of translation (4,5). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (5). eIF4B is thought to assist the eIF4F complex in translation initiation. eIF4H induces the RNA-dependent ATP hydrolysis catalyzed by the initiation factors eIF4A and eIF4B (2,6). eIF4H was further shown to determine the initial rate and extent of eIF4A-mediated mRNA secondary structure unwinding (7). |
| 存放说明 | -20C |
| 参考文献 | 1 . Rogers, G.W. et al. (2001) J Biol Chem 276, 12598-608. 2 . Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847. 3 . Rogers, G.W. et al. (1999) J Biol Chem 274, 12236-44. 4 . Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963. 5 . Svitkin, Y.V. et al. (2001) RNA 7, 382-94. 6 . Richter-Cook, N.J. et al. (1998) J Biol Chem 273, 7579-87. |
Western blot analysis of extracts from HepG2, HeLa and NBT-II cells using eIF4H (D85F2) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells (left) and MCF-7 cells (right) using eIF4H (D85F2) XP® Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® eIF4E siRNA I #6311 or SignalSilence® eIF4E siRNA II #6554 (+), using eIF4E (C46H6) Rabbit mAb #2067 and α-Tubulin (11H10) Rabbit mAb #2125. The eIF4E (C46H6) Rabbit mAb confirms silencing of eIF4E expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of eIF4E siRNA. | |
Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb #2067. 使用eIF4E (C46H6) Rabbit mAb兔单抗 #2067，免疫印迹（Western Blot）分析在不同细胞系提取物。 | |
Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4E (C46H6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from HepG2, HeLa and NBT-II cells using eIF4H (D85F2) XP® Rabbit mAb #3469. 使用eIF4H (D85F2) XP® Rabbit mAb 兔单抗#3469，免疫印迹（Western Blot）分析在HepG2、HeLa和NBT-II细胞系提取物。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF4E (C46H6) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using eIF4G (C45A4) Rabbit mAb #2469. 使用eIF4G (C45A4) Rabbit mAb兔单抗 #2469，免疫印迹（Western Blot）分析在不同细胞系提取物。 | |
Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody #2441. 使用Phospho-eIF4G (Ser1108) Antibody #2441，免疫印迹（Western Blot）分析PC12细胞系提取物，细胞分为对照组（第1泳道），NGF(10 ng/ml)处理（第2泳道），anisomycin (25 µM)处理(第3泳道), U0126 #9903 (10 µM) 处理(第4泳道), Rapamycin #9904处理(100 nM )(第5泳道)和LY294002 #9901处理(25 µM)(第6泳道)。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum, PD98059 or Dexamethasone, using Phospho-eIF4E (Ser209) Antibody #9741 (upper) or eIF4E Antibody #9742 (lower). 使用Phospho-eIF4E (Ser209) Antibody #9741 (上图)和eIF4E Antibody #9742 (下图)，免疫印迹（Western Blot）分析在NIH/3T3细胞系提取物，细胞分为对照组、血清处理组、PD98059处理组或Dexamethasone处理组。 | |
Western blot analysis of extracts from NIH/3T3, 293 and HeLa cells using eIF4B Antibody #3592. 使用eIF4B Antibody #3592，免疫印迹（Western Blot）分析NIH/3T3、293和HeLa细胞系提取物。 | |
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody #3591 (upper) or eIF4B Antibody #3592 (lower). Forty-eight hours following siRNA transfection cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated. 使用Phospho-eIF4B (Ser422) Antibody #3591 (上图)和eIF4B Antibody #3592 (下图)，免疫印迹（Western Blot）分析在HeLa细胞系提取物。细胞在转染siRNA后8小时给予Rapamycin (50 nM)和U0126 (10 µM)处理(如图所示)。 | |
Western blot analysis of extracts from various cell lines using eIF4A1 Antibody #2490. 使用eIF4A1 Antibody #2490，免疫印迹（Western Blot）分析在不同细胞系提取物。 | |
Western blot analysis of extracts from various cell lines using eIF4A (C32B4) Rabbit mAb #2013. 使用eIF4A (C32B4) Rabbit mAb兔单抗 #2013，免疫印迹（Western Blot）分析在不同细胞系提取物。 |
