DUB Antibody Sampler Kit【科瑞奥博】

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DUB Antibody Sampler Kit

货号： 8353T 基本售价： 7504.0 元规格： -

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概述
货号	8353T
描述	The DUB Antibody Sampler Kit提供对精选的去泛素化酶进行鉴定和检测的经济实用抗体组合。这个试剂盒内提供进行至少4次western blot检测的足够磷酸化抗体。 Western Blotting
目标/特异性	CYLD (D1A10) Rabbit mAb recognizes endogenous levels of total CYLD protein. This antibody also detects an unidentified protein of approximately 28 kDa in some cell types. Phospho-CYLD (Ser418) Antibody detects endogenous levels of CYLD protein only when phosphorylated at Ser418. USP9X Antibody recognizes endogenous levels of total USP9X protein and may also cross-react with USP9Y. All other antibodies in this kit detect endogenous levels of total target protein.

性能
供应商	CST
背景	泛素酶（UBEs）催化蛋白质泛素化，去泛素化酶（DUB）在参与相反的过程——去泛素化（1,2）。 CYLD 去泛素化酶调节炎症和细胞增殖，通过去除几个NF-κB信号通路相关蛋白的泛素链下调NF-κB信号通路（3,4）。CYLD Ser418位的磷酸化降低CYLD去泛素化酶的活性，这对于IKKε驱动的转化过程具有重要作用（5）。 STAM-结合蛋白（STAMBP或AMSH）是一种胞内去泛素化酶，具有较强的针对K63位偶联泛素链的泛素异肽酶活性。A20的氨基端包含针对K63位偶联泛素链（如在TRAF6和RIP蛋白上的泛素化修饰）的去泛素活性，，而羧基末端包含针对K48位的泛素连接酶活性，并导致靶蛋白的降解（8）。这两种酶活性都在神经退行性疾病中发挥作用（9-11），调节神经元功能和精子发育（10,13,14）。 UCHL1结合单泛素，而UCHL3结合单泛素和泛素样分子NEDD8（11,12）。 HAUSP能够结合p53并对p53和相关调节蛋白MDM2去泛素化，从而增加这两种蛋白的稳定性（15,16）。 HAUSP的还调节其他泛素化的蛋白质，如叉头转录因子FOXO家族和有丝分裂的应力检查点蛋白CHFR（17,18）。 USP10似乎通过蛋白质相互作用和磷酸化被调节。USP10与RAS-gap SH3结构域结合蛋白（G3BP）相互作用，抑制其拆解泛素链的能力（19）。 ATM介导Thr42和Ser337位的磷酸化能够增加USP10的稳定性，促进其从细胞质再分配到细胞核，它在p53感应基因毒性应激反应后的去泛素化、稳定和激活过程中发挥作用（20）。 USP9X具有保守的具有半胱氨酸肽酶活性的催化结构域，可裂解泛素和多泛素结合物。虽然USP9X表达已被证明对于正常的哺乳动物发育（21-23）至关重要，但其机理还有待阐明。 Application References
存放说明	-20C
参考文献	1 . Nijman, S.M. et al. (2005) Cell 123, 773-86. 2 . Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613. 3 . Regamey, A. et al. (2003) J Exp Med 198, 1959-64. 4 . Glittenberg, M. and Ligoxygakis, P. (2007) Fly (Austin) 1, 330-2. 5 . Hutti, J.E. et al. (2009) Mol Cell 34, 461-72. 6 . McCullough, J. et al. (2006) Curr Biol 16, 160-5. 7 . Kim, M.S. et al. (2006) Biochem Biophys Res Commun 351, 612-8. 8 . Lin, S.C. et al. (2008) J Mol Biol 376, 526-40. 9 . Li, M. et al. (2002) Nature 416, 648-53. 10 . Leroy, E. et al. (1998) Nature 395, 451-2. 11 . Brooks, C.L. et al. (2007) Oncogene 26, 7262-6. 12 . Kurihara, L.J. et al. (2001) Hum Mol Genet 10, 1963-70. 13 . van der Horst, A. et al. (2006) Nat Cell Biol 8, 1064-73. 14 . Osaka, H. et al. (2003) Hum Mol Genet 12, 1945-58. 15 . Oh, Y.M. et al. (2007) Biochem Biophys Res Commun 357, 615-9. 16 . Wada, H. et al. (1998) Biochem Biophys Res Commun 251, 688-92. 17 . Sakurai, M. et al. (2006) J Cell Sci 119, 162-71. 18 . Kwon, J. (2007) Exp Anim 56, 71-7. 19 . Soncini, C. et al. (2001) Oncogene 20, 3869-79. 20 . Yuan, J. et al. (2010) Cell 140, 384-96. 21 . Pantaleon, M. et al. (2001) Mech Dev 109, 151-60. 22 . Noma, T. et al. (2002) Mech Dev 119 Suppl 1, S91-5. 23 . Xu, J. et al. (2005) J Neurosci Res 80, 47-55.

参考图片

Western blot analysis of extracts from Ramos and K-562 cells using UCHL1 (D3T2E) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). As expected, UCHL1 protein expression is not detected in K-562 cells.

Flow cytometric analysis of K-562 cells (blue) and Ramos cells (green) using UCHL1 (D3T2E) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human UCHL1 (hUCHL1-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL3 (hUCHL3-Myc/DDK; +), Myc/DDK-tagged full-length human UCHL5 (hUCHL5-Myc/DDK; +), and Myc/DDK-tagged full-length human BAP1 (hBAP1-Myc/DDK; +), using UCHL1 (D3T2E) XP^® Rabbit mAb (upper) or DYKDDDDK Tag Antibody #2368 (lower).

Western blot analysis of extracts from various cell lines using UCHL1 (D3T2E) XP^® Rabbit mAb (upper) and GAPDH (D16H11) XP^® Rabbit mAb #5174 (lower).

Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using UCHL1 (D3T2E) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using UCHL1 (D3T2E) XP^® Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

Immunohistochemical analysis of paraffin-embedded DU 145 (left; positive) and LNCaP (right; negative) cell pellets using UCHL1 (D3T2E) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using UCHL1 (D3T2E) XP^® Rabbit mAb.

Confocal immunofluorescent analysis of DU 145 (positive; left) and LNCaP (negative; right) cells using UCHL1 (D3T2E) XP^® Rabbit mAb (green). Blue pseudocolor= DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using USP10 (D7A5) Rabbit mAb #8501.

Western blot analysis of extracts from various cell lines using CYLD (D1A10) Rabbit mAb #8462.

Western blot analysis of extracts from various cell lines using UCHL3 (D25E6) Rabbit mAb #8141.

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human USP10 (hUSP10-Myc/DDK, +), using USP10 (D7A5) Rabbit mAb.

Western blot analysis of extracts from various cell lines using USP10 (D7A5) Rabbit mAb.

Confocal immunofluorescent analysis of HCT 116 cells using USP10 (D7A5) Rabbit mAb (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

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DUB Antibody Sampler Kit

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Western Blotting

Application References