| 货号 | 8335T |
| 描述 | The Fatty Acid and Lipid Metabolism Antibody Sampler Kit provides an economical means to evaluate key proteins involved in fatty acid and lipid metabolism. This kit includes enough primary antibody to perform four western miniblot experiments with each primary antibody. |
| 目标/特异性 | AceCS1 (D19C6) Rabbit mAb recognizes endogenous levels of total cytoplasmic acetyl-CoA synthetase. Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody recognizes endogenous levels of ACC only when phosphorylated at Ser79; this antibody recognizes both ACCα and ACCβ. Acetyl-CoA Carboxylase (C83B10) Rabbit mAb recognizes endogenous levels of all isoforms of acetyl-CoA carboxylase protein. Phospho-ATP-Citrate Lyase (Ser455) Antibody recognizes endogenous levels of ATP-citrate lyase only when phosphorylated at Ser455. ACSL1 Antibody, ATP-Citrate Lyase Antibody, Fatty Acid Synthase (C20G5) Rabbit mAb, and Lipin 1 Antibody recognize endogenous levels of their respective target proteins. |
| 供应商 | CST |
| 背景 | The processes of fatty acid and lipid metabolism are vital for cellular nutrient and energy maintenance. Cytoplasmic acetyl-CoA synthetase (AceCS1) catalyzes the conversion of acetate and CoA to acetyl-CoA. Acetyl-CoA synthesized by AceCS1 is used for fatty acid and lipid biosynthesis (1,2). Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). Mammalian long-chain acyl-CoA synthetase (ACSL) catalyzes the ligation of the fatty acid to CoA to form fatty acyl-CoA in a two-step reaction (4). ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholesterol, and acetylcholine, as well as for glucogenesis (5). Phosphorylation of ACL at Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (6). Fatty acid synthase (FAS) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA (7). Lipin 1 plays a role in lipid metabolism in various tissues and cell types including liver, muscle, adipose tissues, and neuronal cell lines (8-10). It has dual functions at the molecular level: Lipin 1 serves as a transcriptional coactivator in the liver and a phosphatidate phosphatase in triglyceride and phospholipid biosynthesis pathways (11).Application References |
| 存放说明 | -20C |
| 参考文献 | 1 . Ikeda, Y. et al. (2001) J Biol Chem 276, 34259-69. 2 . Mashek, D.G. et al. (2004) J Lipid Res 45, 1958-61. 3 . Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33. 4 . Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33. 5 . Luong, A. et al. (2000) J Biol Chem 275, 26458-66. 6 . Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79. 7 . Ha, J. et al. (1994) J Biol Chem 269, 22162-8. 8 . Finck, B.N. et al. (2006) Cell Metab 4, 199-210. 9 . Phan, J. and Reue, K. (2005) Cell Metab 1, 73-83. 10 . Verheijen, M.H. et al. (2003) Genes Dev 17, 2450-64. 11 . Reue, K. and Zhang, P. (2008) FEBS Lett 582, 90-6. |
Western blot analysis of extracts from various cell lines using ACSL1 (D2H5) Rabbit mAb #9189. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded mouse liver using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. | |
Confocal immunofluorescent analysis of 293 cells (all nutrient-starved with Krebs-Ringer bicarbonate buffer for 4 hr), starved only (top left), serum-treated (10%, 30 min; top right), H2O2-treated (10 mM, 10 min; bottom left), or λ phosphatase-treated (2 hr; bottom right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from SH-SY5Y cells, untreated or treated with Oligomycin #9996 (0.5 μM, 30 min), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb (upper) or Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676 (lower). The phospho-specificity of the antibody was verified by λ phosphatase treatment. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded NCI-H2228 cell pellets, untreated (left) or phenformin-treated (right), using Phospho-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb. | |
Western blot analysis of extracts from human white pre-adipocytes and human white adipocytes using Lipin 1 Antibody #5195 (upper) and β-Actin Antibody #4967 (lower). Wetern blot检测人白色脂肪前体细胞和成熟白色脂肪细胞提取物，所用抗体为Lipin 1 Antibody #5195 (上)和β-Actin Antibody #4967(下)。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated for the indicated times, using Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331 (upper) or ATP-Citrate Lyase Antibody #4332 (lower). Wetern blot检测NIH/3T3 细胞提取物，未处理或用PDGF处理相应时间，所用抗体为Phospho-ATP-Citrate Lyase (Ser455) Antibody #4331(上)和ATP-Citrate Lyase Antibody #4332(下)。 | |
Western blot analysis of extracts from various cell lines using Fatty Acid Synthase (C20G5) Rabbit mAb #3180. Wetern blot检测多种细胞系提取物，所用抗体为Fatty Acid Synthase (C20G5) Rabbit mAb 兔单抗#3180。 | |
Western blot analysis of extracts from various cell lines using ATP-Citrate Lyase Antibody #4332. Wetern blot检测多种细胞系提取物，所用抗体为ATP-Citrate Lyase Antibody #4332。 | |
Western blot analysis of extracts from 293 cells, untreated or oligomycin-treated, using Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661. Wetern blot检测293细胞提取物，未处理或用寡霉素处理，所用抗体为Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody #3661。 | |
Western blot analysis of extracts from various cell lines using Acetyl-CoA Carboxylase (C83B10) Rabbit mAb #3676. Wetern blot检测多种细胞系提取物，所用抗体为Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗 #3676。 | |
Western blot analysis of extracts from various cell lines using ACSL1 (D2H5) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using AceCS1 (D19C6) Rabbit mAb #3658. Wetern blot检测多种细胞系提取物，所用抗体为AceCS1 (D19C6) Rabbit mAb 兔单抗#3658。 |
