| 货号 | 9765T |
| 描述 | The Vesicle Trafficking Antibody Sampler kit provides an economical means to analyze proteins involved in the intracellular transport of cargo proteins. This kit includes enough primary and secondary antibody to perform four western blot experiments. |
| 目标/特异性 | Phospho-Caveolin-1 (Tyr14) Antibody detects endogenous levels of Caveolin-1 only when phosphorylated at Tyr14 and does not cross-react with caveolin-2, -3 or caveolin-1β, the short isoform of caveolin-1. Caveolin-1 (D46G3) XP® Rabbit mAb detects endogenous levels of total caveolin-1 protein. Clathrin Heavy Chain (D3C6) XP® Rabbit mAb detects endogenous levels of total clathrin protein. APPL1 (D83H4) XP® Rabbit mAb detects endogenous levels of total APPL1 protein. EEA1 (C45B10) Rabbit mAb detects endogenous levels of total EEA1 protein. Syntaxin 6 (C34B2) Rabbit mAb detects endogenous levels of total Syntaxin 6 protein. GOPC Antibody detects transfected levels of total GOPC protein but can detect endogenous levels of GOPC following IP enrichment. Rab5 (C8B1) Rabbit mAb detects endogenous levels of total Rab5 protein. |
| 供应商 | CST |
| 背景 | Vesicle trafficking is an integral cellular process and the associated proteins involved also play major roles in other signaling pathways. Caveolins are involved in diverse biological functions including vesicular trafficking, cholesterol homeostasis, cell adhesion, apoptosis, and are also indicated in neurodegenerative disease (1). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (2-4). Clathrin-coated vesicles provide for the intracellular transport of proteins following endocytosis and during multiple vesicle trafficking pathways. Vesicles form at specialized areas of the cell membrane where clathrin and associated proteins form clathrin-coated pits. Invagination of these cell membrane-associated pits internalizes proteins and forms an intracellular clathrin-coated vesicle (5,6). Clathrin is the most abundant protein in these vesicles and is present as a basic assembly unit called a triskelion. Each clathrin triskelion is composed of three clathrin heavy chains and three clathrin light chains. Clathrin heavy chain proteins are composed of several functional domains that associate with other vesicle proteins (6). The APPL1 multidomain adaptor protein is a BAR-domain protein family member that is involved in membrane trafficking within a number of signal transduction pathways (7). EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (8,9). Syntaxin 6 is a ubiquitously expressed S25C family member of the SNARE proteins (10,11). Syntaxin 6 protein is localized to the trans-Golgi and within endosomes and regulates membrane trafficking by partnering with a variety of other SNARE proteins (12-14). It has two coiled-coil domains (CC1 and CC2) located in the amino-terminal region and a PDZ domain in the carboxy-terminal region (15). The CC2 domain and its adjacent linker region mediate the association of GOPC with the Golgi protein golgin-160 and the Q-SNARE protein syntaxin 6 (15,16). The PDZ domain of GOPC interacts with the carboxy terminus of target proteins to mediate target protein vesicular trafficking and surface expression (17-20). Rab5 is a member of the Ras superfamily of small Rab GTPases. Rab5 is localized at the plasma membrane and early endosomes and functions as a key regulator of vesicular trafficking during early endocytosis (21). |
| 存放说明 | -20C |
| 参考文献 | 1 . Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304. 2 . Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986. 3 . Rodriguez-Boulan, E. et al. (2005) Nat Rev Mol Cell Biol 6, 233-47. 4 . Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103. 5 . Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75. 6 . Christoforidis, S. et al. (1999) Nature 397, 621-5. 7 . Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17. 8 . Mousavi, S.A. et al. (2004) Biochem J 377, 1-16. 9 . Habermann, B. (2004) EMBO Rep 5, 250-5. 10 . Mu, F.T. et al. (1995) J Biol Chem 270, 13503-11. 11 . Bock, J.B. et al. (2001) Nature 409, 839-41. 12 . Bock, J.B. et al. (1996) J Biol Chem 271, 17961-5. 13 . Wendler, F. and Tooze, S. (2001) Traffic 2, 606-11. 14 . Bock, J.B. et al. (1997) Mol Biol Cell 8, 1261-71. 15 . Mallard, F. et al. (2002) J Cell Biol 156, 653-64. 16 . Charest, A. et al. (2001) J Biol Chem 276, 29456-65. 17 . Hicks, S.W. and Machamer, C.E. (2005) J Biol Chem 280, 28944-51. 18 . Cheng, J. et al. (2002) J Biol Chem 277, 3520-9. 19 . He, J. et al. (2004) J Biol Chem 279, 50190-6. 20 . Wente, W. et al. (2005) J Biol Chem 280, 32419-25. 21 . Ito, H. et al. (2006) Biochem J 397, 389-98. |
Western blot analysis of extracts from various cell lines using GOPC (D10A12) Rabbit mAb #8576. 对多个细胞系使用GOPC (D10A12) Rabbit mAb兔单抗 #8576进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using Syntaxin 6 (C34B2) Rabbit mAb #2869. 对多个细胞系使用Syntaxin 6 (C34B2) Rabbit mAb兔单抗 #2869进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using EEA1 (C45B10) Rabbit mAb #3288. 对多个细胞系使用EEA1 (C45B10) Rabbit mAb兔单抗 #3288进行Western blot分析。 | |
Western blot analysis of extracts from various cell types using APPL1 (D83H4) XP® Rabbit mAb #3858. 对多种细胞系提取物使用APPL1 (D83H4) XP® Rabbit mAb 兔单抗#3858进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb #4796. 对多种细胞系提取物使用Clathrin Heavy Chain (D3C6) XP® Rabbit mAb兔单抗 #4796进行Western blot分析。 | |
对多种细胞系提取物使用Caveolin-1 (D46G3) XP® Rabbit mAb 兔单抗#3267进行Western blot分析。 | |
Western blot analysis of extracts from various cell types using Caveolin-1 (D46G3) XP® Rabbit mAb #3267. | |
Western blotting analysis of extracts from HeLa cells treated with H2O2 for the indicated times using Phospho-Caveolin-1 (Tyr14) Antibody #3251 (upper) or caveolin-1 antibody (lower). 对H2O2按相应时间处理过的HeLa细胞，使用Phospho-Caveolin-1 (Tyr14) Antibody #3251（上图）或caveolin-1抗体（下图）进行Western blot分析。 | |
Western blot analysis of extracts from various cell lines using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb. | |
Confocal immunofluorescent analysis of SH-SY5Y cells using Clathrin Heavy Chain (D3C6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Confocal immunofluorescent analysis of HeLa cells using APPL1 (D83H4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from various cell lines using Rab5 (C8B1) Rabbit mAb. | |
Western blot analysis of extracts from various cell types using APPL1 (D83H4) XP® Rabbit mAb. |
