| 货号 | 9869T |
| 描述 | The Cyclin Antibody Sampler Kit provides an economical means of evaluating the presence of cyclin proteins in cells. The kit contains enough primary and secondary antibodies to perform four western blot experiments with each antibody.Cyclin Antibody Sampler Kit能够经济地评价细胞中细胞周期蛋白的存在。该试剂盒内含足够4次western blot实验的一抗和二抗。 |
| 目标/特异性 | Each antibody in the Cyclin Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other cyclin proteins. |
| 供应商 | CST |
| 背景 | Control of the cell cycle is regulated by a multitude of cellular events and processes. The cyclin dependent kinases (CDK) regulate many of these pathways and constitute an active complex when associated with their cyclin partners. This activity is controlled primarily by phosphorylation, which determines subcellular localization of the CDK/cyclin complex (1,2). Some phosphorylation events control the function of cytoplasmic retention sequences while other events regulate nuclear localization and export sequence function (3,4). Cyclin and cyclin-dependent kinase inhibitor (CKI) levels are regulated by ubiquitination and degradation via the ubiquitin proteasome pathway (5). A variety of CKI proteins associate with these complexes and modulate access to regulatory domains on cyclins (6). Additional complexity is generated as the controlled protein levels of each cyclin oscillate with the stages of cell cycle. Increased expression of Cyclin D1 is associated with certain types of cancer (7,8) and may associate with TSC2 (tuberin) independent of its Cdk partner (9).细胞周期调节受众多的细胞事件和进程控制。细胞周期蛋白依赖性激酶(CDK)调节这些途径，并结合其细胞周期蛋白伙伴，构成一个活化的复合体。该活性主要通过磷酸化来控制，从而决定了CDK/细胞周期蛋白复合物的亚细胞定位(1,2)。有些磷酸化事件控制细胞质滞留序顺序的功能，而其它事件调节核定位和输出顺序功能(3,4)。细胞周期蛋白和细胞周期蛋白依赖性激酶抑制剂(CKI)水平受泛素化和泛素蛋白酶体降解途径的调节(5)。各种CKI蛋白质联合这些复合物并调节-细胞周期蛋白调节结构域的进入(6)。控制随细胞周期阶段振的细胞周期蛋白水平将产生额外的复杂性。细胞周期蛋白D1表达的增加与某些类型的癌症相关(7,8)，且可能联合TSC2（薯球蛋白）而不依赖其Cdk伴侣(9)。Application References |
| 存放说明 | -20C |
| 参考文献 | 1 . Krempler, A. et al. (2005) Cell Mol Life Sci 62, 1379-87. 2 . Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81. 3 . Toyoshima, F. et al. (1998) EMBO J 17, 2728-35. 4 . Sherr, C.J. (1996) Science 274, 1672-7. 5 . Porter, L.A. and Donoghue, D.J. (2003) Prog Cell Cycle Res 5, 335-47. 6 . Benzeno, S. et al. (2006) Oncogene 25, 6291-303. 7 . Diehl, J.A. et al. (1997) Genes Dev 11, 957-72. 8 . Kim, J.K. and Diehl, J.A. (2009) J Cell Physiol 220, 292-6. 9 . Zacharek, S.J. et al. (2005) Cancer Res 65, 11354-60. |
Western blot analysis of extracts from MCF-7, SK-N-MC and HeLa cells, untreated or treated with the proteasome inhibitor MG-132, using Cyclin E2 Antibody #4132.Western blot方法检测MCF-7, SK-N-MC 和 HeLa细胞提取物，分为非处理组合蛋白酶体抑制剂MG-132处理组，使用的抗体为Cyclin E2 Antibody #4132. | |
Flow cytometric analysis of untreated Jurkat cells, using Cyclin A2 (BF683) Mouse mAb versus propidium iodide (DNA content). Note decrease in expression of Cyclin A during late mitosis (arrow). | |
Western blot analysis of extracts from Jurkat and Raji cells using Cyclin H Antibody #2927.Western blot方法检测 Jurkat和Raji 细胞提取物，使用的抗体为 Cyclin H Antibody #2927. | |
Western blot analysis of extracts from various cell lines using Cyclin B1 Antibody #4138.Western blot方法检测不同细胞提取物，使用的抗体为Cyclin B1 Antibody #4138。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with either doxorubicin (0.5 µM, 24 hours) or nocodazole (50 ng/ml, 24 hours), using Cyclin A (BF683) Mouse mAb #4656.Western blot方法检测未处理、阿霉素(0.5 µM)处理24小时计诺考达唑 (50 ng/ml)处理24小时的Hela细胞提取物，使用的抗体为Cyclin A (BF683) Mouse mAb #4656。 | |
Western blot analysis of extracts from SK-N-MC, C6 and mIMCD-3 cells using Cyclin D3 (DCS22) Mouse mAb #2936.Western blot 方法检测SK-N-MC, C6 和mIMCD-3细胞提取物，使用的抗体为Cyclin D3 (DCS22) Mouse mAb #2936。 | |
Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) XP® Rabbit mAb #3741.Western blot方法检测不同细胞提取物，使用的抗体为Cyclin D2 (D52F9) XP® Rabbit mAb #3741。 | |
Western blot analysis of extracts from various cell lines using Cyclin D2 (D52F9) Rabbit mAb. | |
Western blot analysis of extracts from MCF7, L929 and C6 cells using Cyclin D1 (92G2) Rabbit mAb #2978.Western blot方法检测 MCF7, L929 和C6 细胞提取物，使用的抗体为 Cyclin D1 (92G2) Rabbit mAb #2978. | |
Immunohistochemical analysis of paraffin-embedded Apc (min/+) mouse intestine using Cyclin D1 (92G2) Rabbit mAb. | |
Confocal immunofluorescent analysis of HeLa cells using Cyclin B1 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) showing concentrated cyclin B1 staining surrounding mitotic chromatin. | |
Western blot analysis of extracts from various cell types using Cyclin B1 Antibody. | |
Flow cytometric analysis of untreated HT29 cells, using Cyclin D1 (92G2) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Cyclin D1 (92G2) Rabbit mAb in the presence of control peptide (left) or Cyclin D1 Blocking Peptide #1044 (right). | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Cyclin D1 (92G2) Rabbit mAb. |
