| 货号 | 9655T |
| 描述 | The PI3 Kinase Sampler Kit provides an economical means of studying PI3 kinase subunits in cells. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody. |
| 目标/特异性 | Each antibody in the PI3 Kinase Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Each total antibody detects endogenous levels of its target protein. Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody detects endogenous levels of p85/p55 only when phosphorylated at Tyr458/Tyr199. |
| 供应商 | CST |
| 背景 | Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4). |
| 存放说明 | -20C |
| 参考文献 | Cantley, L.C. (2002) Science 296, 1655-7. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80. Stoyanov, B. et al. (1995) Science 269, 690-3. |
Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb #3011. western blot分析MCF-7 和 K-562 细胞提取物，所用抗体为PI3 Kinase p110β (C33D4) Rabbit mAb 兔单抗#3011。 | |
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb #4249. western blot分析Hela细胞和新生小鼠大脑细胞提取物，所用抗体为PI3 Kinase p110α (C73F8) Rabbit mAb兔单抗 #4249。 | |
western blot分析Jurkat 和 NIH/3T3 细胞提取物，所用抗体为PI3 Kinase p85 (19H8) Rabbit mAb 兔单抗#4257。 | |
Western blot analysis of extracts from Jurkat and NIH/3T3 cells using PI3 Kinase p85 (19H8) Rabbit mAb #4257. | |
Western blot analysis of extracts from NIH/3T3-Src cells, untreated or λ-phosphatase-treated, and from C2C12 cells, untreated or H2O2-treated, using Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody #4228. western blot分析NIH/3T3-Src 细胞，未处理组或λ-磷酸酶处理组和C2C12 细胞，未处理组或H2O2处理组，所用抗体为Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody #4228。 | |
Western blot analysis of extracts from 293 and RN33B cells using PI3 Kinase Class III (D4E2) Rabbit mAb. | |
Western blot analysis of extracts from MCF-7 and K-562 cells using PI3 Kinase p110β (C33D4) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using PI3 Kinase p110α (C73F8) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from Jurkat and NIH/3T3 cells, using PI3 Kinase p85 (19H8) Rabbit mAb. | |
Western blot analysis of extracts from HeLa cells and neonatal mouse brain using PI3 Kinase p110α (C73F8) Rabbit mAb. | |
Western blot analysis of extracts from NIH/3T3-Src cells, untreated or treated with lambda phosphatase and from C2C12 cells, untreated or treated with H2O2, using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
