| 货号 | 9769T |
| 描述 | The 14-3-3 Family Antibody Sampler Kit provides an economical means to investigate the expression of various 14-3-3 isoforms within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments. |
| 目标/特异性 | Each antibody in the 14-3-3 Family Antibody Sampler Kit detects endogenous levels of its respective target. |
| 供应商 | CST |
| 背景 | The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes which modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3). |
| 存放说明 | -20C |
| 参考文献 | Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9. Mackintosh, C. (2004) Biochem J 381, 329-42. Dougherty, M.K. and Morrison, D.K. (2004) J Cell Sci 117, 1875-84. Yaffe, M.B. (2002) FEBS Lett 513, 53-7. Bridges, D. and Moorhead, G.B. (2004) Sci STKE 2004, re10. |
Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb #5521. | |
Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb #7413. | |
Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb #5522. | |
Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb. | |
Isoform specificity of 14-3-3γ (D15B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each)were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 γ (D15B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower). | |
Isoform specificity of 14-3-3 η (D23B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each) were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 η (D23B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower). | |
Western blot analysis of extracts from HeLa, NIH/3T3, PC12, and COS cells using 14-3-3 ε Antibody #9635. | |
Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb. | |
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody #9636. | |
Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody. | |
Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody #9638. | |
Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody. |
