mTOR Regulation Antibody Sampler Kit【科瑞奥博】

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mTOR Regulation Antibody Sampler Kit

货号： 9864T 基本售价： 5484.0 元规格： -

产品信息

概述
货号	9864T
描述	The mTOR Regulation Sampler Kit provides an economical means to evaluate the regulation of mTOR signaling by such proteins as phosphorylated Raptor, RagC and PRAS40. The kit contains enough primary and secondary antibodies to perform four Western blot experiments per primary antibody.
目标/特异性	Each antibody in the mTOR Regulation Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. Phospho-Raptor (Ser792) Antibody may also detect non-specific signals of various molecular weights.

性能
供应商	CST
背景	The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).
存放说明	-20C
参考文献	Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. Brown, E.J. et al. (1994) Nature 369, 756-8. Sabatini, D.M. et al. (1994) Cell 78, 35-43. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. Dennis, P.B. et al. (2001) Science 294, 1102-5. Fang, Y. et al. (2001) Science 294, 1942-5. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7. Hara, K. et al. (2002) Cell 110, 177-89. Kim, D.H. et al. (2002) Cell 110, 163-75. Beugnet, A. et al. (2003) J Biol Chem 278, 40717-22. Nojima, H. et al. (2003) J Biol Chem 278, 15461-4. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23. Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. Wullschleger, S. et al. (2006) Cell 124, 471-84. Sancak, Y. et al. (2008) Science 320, 1496-501. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.

参考图片

Confocal immunofluorescent analysis of mouse embryonic fibroblast (MEF) cells using mTOR (7C10) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human lymphoma using RagC (D31G9) XP^® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using RagC (D31G9) XP^® Rabbit mAb.

Western blot analysis of extracts from serum-starved NIH/3T3 cells, untreated or insulin-treated (150 nM, 5 minutes), alone or in combination with λ-phosphatase, using Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb (upper) or mTOR (7C10) Rabbit mAb #2983.

Confocal immunofluorescent analysis of HeLa cells, rapamycin-treated (#9904, 10 μM for 2 hours, left), insulin-treated (150 nM for 6 minutes, middle) or insulin- and λ-phosphatase-treated (right), using Phospho-mTOR (Ser2448) (D9C2) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® mTOR siRNA II (+), using mTOR (7C10) Rabbit mAb #2983 and α-Tubulin (11H10) Rabbit mAb #2125. mTOR (7C10) Rabbit mAb confirms silencing of mTOR expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of mTOR siRNA.

Western blot analysis of extracts from various cell types using PRAS40 (D23C7) XP® Rabbit mAb #2691.

western blot 分析不同细胞系提取物，所用抗体为PRAS40 (D23C7) XP® Rabbit mAb 兔单抗#2691.

Western blot analysis of extracts from serum starved H3255, Mkn45 and NIH/3T3 cells, untreated or treated with either Iressa (1 μM, 3 hours), Su11274 (1 μM, 3 hours) or insulin (150 nM, 15 minutes), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997 (upper) or PRAS40 (D23C7) Rabbit mAb #2691 (lower).

western blot 分析血清饥饿的H3255, Mkn45 和NIH/3T3细胞提取物，未处理组和Iressa (1 μM, 3 小时), Su11274 (1 μM, 3 小时)或胰岛素 (150 nM, 15 分钟)处理组。所用抗体为 Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997兔单抗 (上) 或 PRAS40 (D23C7) Rabbit mAb兔单抗 #2691 (下)

Western blot分析C2C12 或293 cells，未处理组或AICAR (0.5 mM for 30 分钟) 或 oligomycin (0.5 μM for 30 分钟)处理组，所用抗体为Phospho-Raptor (Ser792) Antibody #2083 (上和左下图) 或 Raptor Antibody #2280 (上和右下图). *与200 kDa的条带发生交叉反应。

Western blot analysis of C2C12 or 293 cells, untreated or treated with AICAR (0.5 mM for 30 minutes) or oligomycin (0.5 μM for 30 minutes), using Phospho-Raptor (Ser792) Antibody #2083 (upper and lower left) or Raptor Antibody #2280 (upper and lower right). *Cross-reacting bands at 200 kDa.

Western blot analysis of extracts from various cell types using mTOR (7C10) Rabbit mAb #2983.western blot 分析不同细胞系提取物，所用抗体为mTOR (7C10) Rabbit mAb #2983。

Western blot analysis of extracts from serum starved H3255, Mkn45 and NIH/3T3 cells, untreated or treated with either Iressa (1 μM, 3 hours), Su11274 (1 μM, 3 hours) or insulin (150 nM, 15 minutes), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 (D23C7) Rabbit mAb #2691 (lower).

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with insulin (100 nM, 5 minutes) or with insulin and λ phosphatase, using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).