| 货号 | 9868T |
| 描述 | The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform four western blot experiments.CDK Antbody Sampler Kit能够经济地评价Cdk蛋白。该试剂盒内含足够4次western blot实验的一抗和二抗。 |
| 目标/特异性 | Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members. |
| 供应商 | CST |
| 背景 | Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).周期素依赖性蛋白激酶(CDKs)是细胞周期进程的核心效应器。CDK的活性通过与其细胞周期蛋白伴侣和周期蛋白依赖性激酶抑制剂(CKIs)相互作用而被调节，也可通过激活和抑制磷酸化事件。抑制效应由能够靶向CDK1氨基末端残基的Wee1和Myt1介导(1,2)。Cdc25磷酸酶去磷酸化这些残基将导致CDK激酶活性的激活(3)。CDK7/cyclinH络合物是普遍存在的哺乳动物CDK活化激酶(CAK)，能够磷酸化CDKs中T-回路结构域的保守苏氨酸残基。 RNA聚合酶II的羧基端结构域也是CAK以及CDK9/cyclinT的靶标(4,5)。CDK4/ 6联和Cyclin D并磷酸化视网膜母细胞瘤蛋白，通过G1期限制点启动周期进展(6)。CDK2能够联合早S期的cyclinE和G2后期的cyclinA。CDK1/cyclinB调节有丝分裂事件的启动(7)。Application References |
| 存放说明 | -20C |
| 参考文献 | 1 . Fattaey, A. and Booher, R.N. (1997) Prog Cell Cycle Res 3, 233-40. 2 . Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228. 3 . De Falco, G. and Giordano, A. Cancer Biol Ther 1, 342-7. 4 . Kaldis, P. (1999) Cell Mol Life Sci 55, 284-96. 5 . Booher, R.N. et al. (1997) J Biol Chem 272, 22300-6. 6 . Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91. 7 . Sherr, C.J. (1995) Trends Biochem Sci 20, 187-90. |
Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb #12790. | |
Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb #9116. | |
Flow cytometric analysis of Jurkat cells using CDK4 (D9G3E) Rabbit mAb and Propidium Iodid (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. | |
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb #13331. | |
Confocal immunofluorescent analysis of HT-29 cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left), SignalSilence® cdc2 siRNA I #3500 (center) or SignalSilence® cdc2 siRNA II #3600 (right), using cdc2 (POH1) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb. | |
Immunoprecipitation of CDK6 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CDK6 (D4S8S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CDK6 (DCS83) Mouse mAb #3136. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK4 (D9G3E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CDK4 (D9G3E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). | |
Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb. | |
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using CDK9 (C12F7) Rabbit mAb #2316.Western blot方法检测HeLa, NIH/3T3 和C6 细胞提取物，使用的抗体为CDK9 (C12F7) Rabbit mAb #2316。 | |
Confocal immunofluorescent analysis of MCF7 cells using CDK4 (D9G3E) Rabbit mAb (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8493 (red), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #3458 (blue pseudocolor). | |
Western blot analysis of extracts from various cell types using CDK2 (78B2) Rabbit mAb #2546.Western blot 检测不同细胞提取物，使用的抗体为CDK2 (78B2) Rabbit mAb #2546。 | |
Western blot analysis of extracts from SK-N-MC and C6 cells using CDK7 (MO1) Mouse mAb #2916.Western blot方法检测SK-N-MC和 C6细胞提取物，使用的抗体为 CDK7 (MO1) Mouse mAb #2916。 | |
Immunohistochemical analysis of paraffin-embedded K7M2 mouse syngeneic tumor using CDK9 (C12F7) Rabbit mAb. |
