TRAF Antibody Sampler Kit【科瑞奥博】

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TRAF Antibody Sampler Kit

货号： 8347T 基本售价： 4018.0 元规格： -

产品信息

概述
货号	8347T
描述	The TRAF Antibody Sampler Kit provides an economical means to evaluate endogenous levels of TRAF1, 2, 3, and 6. The kit contains enough primary and secondary antibodies to perform four western mini-blot experiments.
目标/特异性	TRAF1 (45D3) Rabbit mAb detects endogenous levels of total TRAF1 protein. TRAF2 (C192) Antibody detects endogenous levels of total human TRAF2 protein. TRAF3 Antibody detects endogenous levels of total TRAF3 protein. TRAF6 (D21G3) Rabbit mAb detects endogenous levels of total TRAF6 protein. Cross-reactivity was not detected with other family members at physiological conditions.

性能
供应商	CST
背景	TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain" which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.
存放说明	-20C
参考文献	Arch, R.H. et al. (1998) Genes Dev. 12, 2821-30. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-88. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91. Rothe, M. et al. (1994) Cell 78, 681-92. Yeh, W.C. et al. (1997) Immunity 7, 715-25. Reinhard, C. et al. (1997) EMBO J 16, 1080-92. Rothe, M. et al. (1995) Science 269, 1424-7. Wu, H. and Arron, J.R. (2003) Bioessays 25, 1096-105. Lomaga, M.A. et al. (1999) Genes Dev 13, 1015-24. Lomaga, M.A. et al. (2000) J Neurosci 20, 7384-93. Ye, H. et al. (2002) Nature 418, 443-7. Cao, Z. et al. (1996) Nature 383, 443-6. Muzio, M. et al. (1997) Science 278, 1612-5. Medzhitov, R. et al. (1998) Mol Cell 2, 253-8. Ishida, T. et al. (1996) J Biol Chem 271, 28745-8. Darnay, B.G. et al. (1998) J Biol Chem 273, 20551-5. Wong, B.R. et al. (1998) J Biol Chem 273, 28355-9. Khursigara, G. et al. (1999) J Biol Chem 274, 2597-600. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. Wong, B.R. et al. (1999) Mol Cell 4, 1041-9.

参考图片

Western blot analysis of extracts from various cell lines using TRAF6 (D21G3) Rabbit mAb.

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with a cDNA expression construct encoding full-length human TRAF6 (+), using TRAF6 (D21G3) Rabbit mAb.

Western blot analysis of extracts from various cell lines using TRAF6 (D21G3) Rabbit mAb #8028. Western blot 检测多种细胞系提取物，使用抗体为TRAF6 (D21G3) Rabbit mAb #8028。

Western blot analysis of extracts from Raji and SR cells using TRAF1 (45D3) Rabbit mAb #4715. Western blot 检测Raji和SR 细胞提取物，使用抗体为TRAF1 (45D3) Rabbit mAb #4715。

Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with a human TRAF3 construct (+), using TRAF3 Antibody #4729. Western blot 检测 COS-7细胞提取物，未转染(-)或人TRAF3转染 (+) ；使用抗体为TRAF3 Antibody #4729。

Western blot analysis of extracts from various cell lines using TRAF2 (C192) Antibody #4724. Western blot 检测多种细胞系提取物，使用抗体为TRAF2 (C192) Antibody #4724。

Confocal immunofluorescent analysis of HT-1080 (upper) and HEK/293 (lower) cells, untreated (left) or treated with TNF-α #8902 (right), using TRAF1 (45D3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using TRAF3 Antibody.

Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with human TRAF3 construct (+) using TRAF3 Antibody.

Western blot analysis of extracts from Jurkat, 293, HeLa, C2C12, and NIH/3T3 cells, using TRAF2 (C192) Antibody.

Immunohistochemical analysis of paraffin-embedded human tonsil using TRAF1 (45D3) Rabbit mAb preincubated with a control peptide (left) or TRAF1 Blocking Peptide #1066 (right).

Flow cytometric analysis of Raji cells, using TRAF1 (45D3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Western blot analysis of extracts from Raji and SR cells, using TRAF1 (45D3) Rabbit mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.