| 货号 | 9966T |
| 描述 | The IKK Isoform Antibody Sampler Kit provides an economical means to investigate NFkappaB signaling within the cell. The kit contains primary and secondary antibodies to perform four Western blots with each antibody. |
| 目标/特异性 | IKKα, IKKβ, IKKγ, and IKKε antibodies detect endogenous levels of total IKKα, IKKβ, IKKγ and IKKε proteins, respectively. These antibodies do not cross-react with IKK subunits other than their specified target. |
| 供应商 | CST |
| 背景 | The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation; Ser177 and Ser181 in the activation loop of IKKβ (serine 176 and 180 in IKKα) are the specific sites whose phosphorylation causes conformational changes resulting in kinase activation (10-13). |
| 存放说明 | -20C |
| 参考文献 | Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. Brown, K. et al. (1995) Science 267, 1485-8. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Chen, Z.J. et al. (1996) Cell 84, 853-62. Zandi, E. et al. (1997) Cell 91, 243-52. Karin, M. (1999) Oncogene 18, 6867-74. DiDonato, J.A. et al. (1997) Nature 388, 548-54. Mercurio, F. et al. (1997) Science 278, 860-6. Johnson, L.N. et al. (1996) Cell 85, 149-58. Delhase, M. et al. (1999) Science 284, 309-13. |
Western blot analysis of extracts from various cell lines using IKKα (3G12) Mouse mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Flow cytometric analysis of HCT 116 cells using IKKα (3G12) Mouse mAb (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red). Anti-mouse IgG (H+L), F(ab)2Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody. | |
Confocal immunofluorescent analysis of HCT 116 (high expression; left) and IGROV-1 (low expression; right) cells using IKKα (3G12) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from wild-type, IKKα (-/-), and IKKβ (-/-) mouse embryonic fibroblasts (MEFs) using IKKβ (D30C6) Rabbit mAb (upper) and GAPDH (14C10) Rabbit mAb #2118 (lower). | |
Western blot analysis of extracts from various cell lines using IKKβ (D30C6) Rabbit mAb. | |
Western blot analysis of recombinant IKKε Kinase #7553 and Raw 264.7 cells, untreated or LPS-treated, using IKKε Antibody #2690.Western免疫印迹。用IKKε Antibody #2690研究未经处理的和经LPS处理的重组IKKε 激酶#7553 和 Raw 264.7细胞的细胞提取液。 | |
Western blot analysis of recombinant IKKε Kinase #7553 and RAW 264.7 cells, untreated or LPS-treated, using IKKε Antibody. | |
Western blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells using IKKβ Antibody #2684.Western免疫印迹。用IKKβ Antibody #2684研究NIH/3T3, HeLa和PC12 细胞的细胞提取液。 | |
Western blot analysis of extracts from various cell types using IKKγ Antibody #2685.Western免疫印迹。用IKKγ Antibody #2685研究各种细胞的细胞提取液。 | |
Western blot analysis of extracts from NIH/3T3, HeLa and PC12 cells using IKKα Antibody #2682.Western免疫印迹。用IKKα Antibody #2682研究NIH/3T3, HeLa和PC12 细胞的细胞提取液。 | |
Western blot analysis of extracts from HeLa cells, untreated or treated with TNF-α (20 ng/ml) and Calyculin A #9902 (50 nM), using Phospho-IKKγ (Ser376) Antibody #2689 (top) or IKKγ (DA10-12) Mouse mAb (bottom). | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and membrane localization, using IKKγ Antibody. | |
Western blot analysis of extracts from various cell lines using IKKγ Antibody. | |
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition. |
