Human TNF-alpha MAb (Clone 28401R) (1 MG)【科瑞奥博】

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Human TNF-alpha MAb (Clone 28401R) (1 MG)

货号： MAB610R-01M 基本售价： 19254.0 元规格： 1 mg

产品信息

概述
货号	MAB610R-01M
简介	Recombinant Monoclonal Antibody
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),Neutralization
目标/特异性	Detects human TNF-alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human TNF‑ beta, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL Neutralization: Measured by its ability to neutralize TNF-alpha -induced cytotoxicity in the L-929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.01-0.04 μg/mL in the presence of 0.75 ng/mL Recombinant Human TNF-alpha and 1 μg/mL actinomycin D.
来源	Recombinant Monoclonal Mouse IgG₁ Clone # 28401R
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	E. coli-derived recombinant human TNF-alpha Gly57-Leu233 Accession # P01375
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
背景	TNF-alpha, designated TNFSF2, is a trimeric glycoprotein active in bothmembrane bound and secreted forms. TNF-alpha is produced by severallymphoid cells as well as by astrocytes, endothelial cells, and smoothmuscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtuallyall cell types where it triggers the activation of multiple signaltransduction pathways and modulates the expression of a wide variety ofgenes.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Cancer Biomarkers Conventional/Classical Dendritic Cells Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction gamma delta T Cells ICC / IHC Images: TNF-alpha Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Interactive Pathway: Adipocytokines & Insulin Signaling Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Natural Killer T (NKT) Cells Neuroinflammation Obesity Plasmacytoid Dendritic Cells Preeclampsia Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest Th1 Cells Th22 Cells TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-alpha (Catalog #
210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Recombinant Monoclonal Antibody (Catalog # MAB610R). The ND₅₀ is typically
0.01-0.04 μg/mL in the presence of the metabolic inhibitor actinomycin D (1 μg/mL).