| 货号 | MAB603R-01M |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor | 全称 | Interleukin 3 |
| 应用 | ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects human IL-3 in ELISAs and Western blots. In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, recombinant mouse (rm) IL-1 beta, rhIL-2, rhIL-3 R alpha, rhIL-3 R beta, rmIL-3, rhIL-4, rmIL-4, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, bovine FGF acidic, bovine FGF basic, rhG-CSF, rhGM-CSF, rmGM-CSF, rhLIF, hPDGF, porcine PDGF, human TGF‑ beta 1, porcine TGF‑ beta 1, rhTGF‑ beta 1, porcine TGF‑ beta 2, porcine TGF‑ beta 1.2, rhTNF-alpha, or rhTNF-beta was observed. Does not neutralize the biological activity of rmIL‑3. |
| 使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑3-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑3. |
| 来源 | Recombinant Monoclonal Mouse IgG1 Clone # 4815R |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3562 (Human); 16187 (Mouse); 24495 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1. | |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant human IL-3 Ala20-Phe152 Accession # P08700 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells. IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of at least two subunits, an IL-3 specific alpha chain which binds IL-3 with low affinity and a common beta chain that is shared by the IL-5 and GM-CSF high-affinity receptors. Although the beta chain itself does not bind IL-3, it confers high-affinity IL-3 binding in the presence of the alpha chain. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by IL-3 and Neutralization by Human IL-3 Antibody. Recombinant Human IL-3 (Catalog # 203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-3 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Recombinant Monoclonal Antibody (Catalog # MAB603R). The ND50 is typically 0.1-0.3 µg/mL. |
