Human/Mouse IL-5 MAb (Clone TRFK5R) ( )【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human/Mouse IL-5 MAb (Clone TRFK5R) ( )

货号： MAB405R-01M 基本售价： 19254.0 元规格： 1 mg

产品信息

概述
货号	MAB405R-01M
简介	Recombinant Monoclonal Antibody
别名	BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
全称	Interleukin 5
应用	ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 4-15 ng/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑5.
来源	Recombinant Monoclonal Rat IgG₁ Clone # TRFK5R
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1. Authors: Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E PLoS ONE, 2014;9(2):e90298. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from cell culture supernatant
免疫原	Mouse T cell clone-derived partially purified recombinant mouse IL-5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Mouse
标记	Unconjugated
背景	IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Common beta Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells Granulocyte Markers Group 2 ILCs ICC / IHC Images: IL-5 Inflammatory Mediators Mini-review: Interleukin 5 T Cell Cytokine Signaling Th2 Cells

参考图片

Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405R). The ND₅₀ is typically 4-15 ng/mL.