| 货号 | MAB275R-01M |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha |
| 应用 | Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects human and primate CXCL1/GRO alpha /KC/CINC-1 in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 0.25 µg/106cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL1/GRO alpha /KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 10 ng/mL Recombinant Human CXCL1/GRO alpha /KC/CINC‑1. |
| 来源 | Recombinant Monoclonal Mouse IgG2B Clone # 20326R |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2919 (Human); 14825 (Mouse); 81503 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant human CXCL1/GRO alpha /KC/CINC-1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human, Primate |
| 标记 | Unconjugated |
| 背景 | The gene for CXCL1/GRO alpha was initially discovered in hamster cells, using subtractive hybridization techniques, as a message that is over-expressed in tumorigenic cells and in normal cells during growth stimulation. The hamster cDNA was cloned and used as a probe for the subsequent cloning of the human GRO cDNA. Independently, a cDNA encoding a secreted protein with melanoma growth stimulating activity (MGSA) was also cloned from a human melanoma cell line and found to be identical to GRO. In addition to the initially cloned GRO gene, now designated CXCL1, two additional GRO genes, GRO beta or MIP-2 alpha and GRO gamma or MIP‑2 beta, which shared 90% and 86% amino acid sequence homology, respectively, with CXCL1, have been identified. All three human GROs are members of the alpha (C-X-C) subfamily of chemokines. The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes, and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B. It remains to be seen if a unique GRO receptor(s) also exist. The rat homolog of human CXCL1, CINC, is much more active than human CXCL1 on rat neutrophils, suggesting that this cytokine may have selective species specificity. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of CXCL1/GRO alpha /KC/CINC‑1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 1 μg/mL LPS for 24 hours and 3 μM monensin for 2 hours were stained with Recombinant Mouse Anti-Human/Primate CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB275R, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. | |
Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCL1/GRO alpha Antibody. Recombinant Human CXCL1/GRO alpha /KC/CINC-1 (Catalog # 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/ GRO alpha /KC/CINC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human/Primate CXCL1/GRO alpha /KC/CINC-1 Monoclonal Antibody (Catalog # MAB275R). The ND50 is typically 3-15 µg/mL. |
